ID   Y1196_CLOPE             Reviewed;         662 AA.
AC   Q8XL46;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=UPF0313 protein CPE1196 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=CPE1196;
OS   Clostridium perfringens (strain 13 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T.,
RA   Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-
RT   eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; BA000016; BAB80902.1; -; Genomic_DNA.
DR   RefSeq; WP_011010317.1; NC_003366.1.
DR   AlphaFoldDB; Q8XL46; -.
DR   SMR; Q8XL46; -.
DR   STRING; 195102.gene:10490459; -.
DR   EnsemblBacteria; BAB80902; BAB80902; BAB80902.
DR   KEGG; cpe:CPE1196; -.
DR   HOGENOM; CLU_018288_2_0_9; -.
DR   OMA; TCMYYTG; -.
DR   Proteomes; UP000000818; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..662
FT                   /note="UPF0313 protein CPE1196"
FT                   /id="PRO_0000076382"
FT   DOMAIN          296..567
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          597..662
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        614..654
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         310
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         314
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         317
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   662 AA;  76125 MW;  CB6C0C3C808770B8 CRC64;
     MSENKFLPIC KDDMIERGWE QCDFVLVTAD AYIDHHSFGT AIISRVLENA GYKVGIIAQP
     DWKSVDDFKK LGRPRLGFLV NGGNMDPMVN HYTVSKKLRK KDLYTPKGEM GKRPDRATIV
     YCNKIREAYK DVNIVIGGIE ASLRRFAHYD YWENKVRKSI LVDSGADLLV YGMSEKQIVE
     VADFLNQGFD GKYIRHIPGT CYIADSLDEI YEEHIVLPSF KEVSSDKRTY AECFKIQYDE
     QDPVRGRTLV QEHNGKYVVI NKPEMPLSRE ELDRVYALPY QKTYHPIYEK DGGIAAIEEV
     KFSLVSSRGC SGNCSFCAIT FHQGRIVTSR SEDSIVEEAE EITKYDDFKG YIHDIGGPTA
     NFRKPACKKQ LTLGACKHKR CMSPGICKNM EVDHREYLHL LRRVRKLPGI KKVFIRSGLR
     YDYIMADKDD TFFKELVEHH VSGQLKVAPE HVSPNVLKYM GKPAGKTYDE FRRKFFRITE
     RLGKKQFIIP YLMSSHPGCK LEDAIMLAEY LRDINYQPEQ VQDFYPTPGT LSTTMFYTGL
     DPLTMEEVYI PRSKEEKAMQ RALLQFKNPK NYNIVYDALV KAGREDLIGN GPKCLIRDKN
     SFGKGNNHSN HKSGGRKNRN ENSGRRESED KKRSSHSKKQ RGNKSRGFDQ KSQRGSKGKK
     RR