ID   Y1205_PSEAE             Reviewed;         315 AA.
AC   Q9I4D3;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Putative quercetin 2,3-dioxygenase PA1205;
DE            Short=Putative quercetinase;
DE            EC=1.13.11.24;
DE   AltName: Full=Pirin-like protein PA1205;
GN   OrderedLocusNames=PA1205;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Putative quercetin 2,3-dioxygenase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6-
CC         dihydroxybenzoate + CO; Xref=Rhea:RHEA:15381, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17245, ChEBI:CHEBI:57628, ChEBI:CHEBI:57694;
CC         EC=1.13.11.24;
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 divalent metal cation. {ECO:0000250};
CC   -!- PATHWAY: Flavonoid metabolism; quercetin degradation.
CC   -!- SIMILARITY: Belongs to the pirin family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04594.1; -; Genomic_DNA.
DR   PIR; A83496; A83496.
DR   RefSeq; NP_249896.1; NC_002516.2.
DR   RefSeq; WP_003112455.1; NZ_QZGE01000006.1.
DR   AlphaFoldDB; Q9I4D3; -.
DR   SMR; Q9I4D3; -.
DR   STRING; 287.DR97_729; -.
DR   PaxDb; Q9I4D3; -.
DR   PRIDE; Q9I4D3; -.
DR   DNASU; 882088; -.
DR   EnsemblBacteria; AAG04594; AAG04594; PA1205.
DR   GeneID; 882088; -.
DR   KEGG; pae:PA1205; -.
DR   PATRIC; fig|208964.12.peg.1251; -.
DR   PseudoCAP; PA1205; -.
DR   HOGENOM; CLU_045717_1_0_6; -.
DR   InParanoid; Q9I4D3; -.
DR   OMA; RPHPHIN; -.
DR   PhylomeDB; Q9I4D3; -.
DR   BioCyc; PAER208964:G1FZ6-1230-MON; -.
DR   UniPathway; UPA00724; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008127; F:quercetin 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR012093; Pirin.
DR   InterPro; IPR008778; Pirin_C_dom.
DR   InterPro; IPR003829; Pirin_N_dom.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR13903; PTHR13903; 1.
DR   Pfam; PF02678; Pirin; 1.
DR   Pfam; PF05726; Pirin_C; 1.
DR   PIRSF; PIRSF006232; Pirin; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   3: Inferred from homology;
KW   Dioxygenase; Metal-binding; Oxidoreductase; Reference proteome.
FT   CHAIN           1..315
FT                   /note="Putative quercetin 2,3-dioxygenase PA1205"
FT                   /id="PRO_0000214068"
FT   BINDING         77
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
FT   BINDING         123
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   315 AA;  34455 MW;  1AB4225EB18C63BD CRC64;
     MSNTEERCDL SSSRDCPERH EHLLQRVTAR AAEIGGGITV SRLMPSRQRR MIGAWCFLDH
     AGPAEFEPGG GLAVGPHPHI GLQTFTWMIQ GEALHRDSLG NVQVIRPGQV NLMTAGHGIA
     HTEESLPDER HAHAAQLWIA LPYEQRDIAP AFDHHPDLPR WQEQGVTFTL LAGALAGRQA
     PCRLYSPLLG ADLACHDAST LQLTLDPHFE YGLLPLEGGL EVGGEHFAVN ELAYLGDGRD
     GLQLQLDPGA RVLLLGGAPF GAEIFMWWNF VGHSKGEIAR AQKAWEEGDA RFGRLDALEG
     PRLSAPPIPW KIDAE