Y1210_PSEAE
ID Y1210_PSEAE Reviewed; 232 AA.
AC Q9I4C8;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Putative quercetin 2,3-dioxygenase PA1210;
DE Short=Putative quercetinase;
DE EC=1.13.11.24;
DE AltName: Full=Pirin-like protein PA1210;
GN OrderedLocusNames=PA1210;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Putative quercetin 2,3-dioxygenase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6-
CC dihydroxybenzoate + CO; Xref=Rhea:RHEA:15381, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17245, ChEBI:CHEBI:57628, ChEBI:CHEBI:57694;
CC EC=1.13.11.24;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC Note=Binds 1 divalent metal cation. {ECO:0000250};
CC -!- PATHWAY: Flavonoid metabolism; quercetin degradation.
CC -!- SIMILARITY: Belongs to the pirin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG04599.1; -; Genomic_DNA.
DR PIR; D83493; D83493.
DR RefSeq; NP_249901.1; NC_002516.2.
DR RefSeq; WP_003086661.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; Q9I4C8; -.
DR SMR; Q9I4C8; -.
DR STRING; 287.DR97_724; -.
DR PaxDb; Q9I4C8; -.
DR PRIDE; Q9I4C8; -.
DR DNASU; 881966; -.
DR EnsemblBacteria; AAG04599; AAG04599; PA1210.
DR GeneID; 881966; -.
DR KEGG; pae:PA1210; -.
DR PATRIC; fig|208964.12.peg.1256; -.
DR PseudoCAP; PA1210; -.
DR HOGENOM; CLU_064194_2_2_6; -.
DR InParanoid; Q9I4C8; -.
DR OMA; NLRVWND; -.
DR PhylomeDB; Q9I4C8; -.
DR BioCyc; PAER208964:G1FZ6-1235-MON; -.
DR UniPathway; UPA00724; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008127; F:quercetin 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR012093; Pirin.
DR InterPro; IPR003829; Pirin_N_dom.
DR InterPro; IPR041602; Quercetinase_C.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR43212; PTHR43212; 1.
DR Pfam; PF02678; Pirin; 1.
DR Pfam; PF17954; Pirin_C_2; 1.
DR PIRSF; PIRSF006232; Pirin; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 3: Inferred from homology;
KW Dioxygenase; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..232
FT /note="Putative quercetin 2,3-dioxygenase PA1210"
FT /id="PRO_0000214069"
FT BINDING 57
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 59
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 101
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
SQ SEQUENCE 232 AA; 25912 MW; E2958AC8D71A0E5D CRC64;
MIERRPFDRL GHANHGWLNA RHHFSFADYY DPEREDWGRL RVWNDDEIAA GSGFPPHPHR
DMEIITYVRE GAITHQDSLG NKGRTEAGDV QVMSAGTGIV HSEYNLEAET TRIFQIWIIP
DRRGDQPRWG SKPFPKAERD GRFVTLASGD EQDSEALHIR ADAEVAAVTL KAGQSAEYAL
ENGRRAYLVP ATGSIEVNGV RAEARDGLAV RDEPTLKVTA LEDSEVLLVE VA