ID   Y1216_CLOPS             Reviewed;         662 AA.
AC   Q0STL9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=UPF0313 protein CPR_1216 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=CPR_1216;
OS   Clostridium perfringens (strain SM101 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=289380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SM101 / Type A;
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T.,
RA   Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H.,
RA   Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A.,
RA   Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D.,
RA   Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J.,
RA   Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B.,
RA   Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial pathogen,
RT   Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; CP000312; ABG86957.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0STL9; -.
DR   EnsemblBacteria; ABG86957; ABG86957; CPR_1216.
DR   KEGG; cpr:CPR_1216; -.
DR   OMA; TCMYYTG; -.
DR   BioCyc; CPER289380:GI76-1232-MON; -.
DR   Proteomes; UP000001824; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..662
FT                   /note="UPF0313 protein CPR_1216"
FT                   /id="PRO_1000067194"
FT   DOMAIN          296..567
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          597..662
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        614..654
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         310
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         314
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         317
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   662 AA;  76156 MW;  DC14F51D322A8F3F CRC64;
     MSENKFLPIC KDDMIERGWE QCDFVLVTAD AYIDHHSFGT AIISRVLENA GYKVGIIAQP
     DWKSVDDFKK LGRPRLGFLV NGGNMDPMVN HYTVSKKLRK KDLYTPKGEM GKRPDRATIV
     YCNKIREAYK DVNIVIGGIE ASLRRFAHYD YWDNKVRKSI LVDSGADLLV YGMSEKQIVE
     VADFLNQGFD GKYIRHIPGT CYIADSLDEI YEEHIVLPSF KDVSSDKRTY AECFKIQYDE
     QDPVRGRTLV QEHNGKYVVI NKPEMPLSRE ELDRVYALPY QKTYHPIYEK DGGIAAIEEV
     KFSLVSSRGC SGNCSFCAIT FHQGRIVTSR SEDSIVEEAE EITKYDDFKG YIHDIGGPTA
     NFRKPACKKQ LTLGACKHKR CMSPGICKNM EVDHREYLHL LRRVRKLPGI KKVFIRSGLR
     YDYIMADKDD TFFKELVEHH VSGQLKVAPE HVSPNVLKYM GKPAGKTYDE FRRKFFKITE
     RLGKKQFIIP YLMSSHPGCK LEDAIMLAEY LRDINYQPEQ VQDFYPTPGT LSTTMFYTEL
     DPLTMEEVYI PRSKEEKAMQ RALLQFKNPK NYNIVYDALV KAGREDLIGN GPKCLIRDKN
     SFGKGNNHSN HKSGGRKSRN ENSGRRESED KKRSSHSKKQ RGNKSRGFDQ KSQRVSKGKK
     RR