CAPSD_AMVLE
ID CAPSD_AMVLE Reviewed; 221 AA.
AC P03591;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Capsid protein;
DE Short=CP;
DE AltName: Full=Coat protein;
GN ORFNames=ORF3b;
OS Alfalfa mosaic virus (strain 425 / isolate Leiden).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Bromoviridae; Alfamovirus.
OX NCBI_TaxID=12322;
OH NCBI_TaxID=4045; Apium graveolens (Celery).
OH NCBI_TaxID=83862; Astragalus glycyphyllos (Wild liquorice).
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=41386; Caryopteris incana.
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH NCBI_TaxID=35936; Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH NCBI_TaxID=3864; Lens culinaris (Lentil) (Cicer lens).
OH NCBI_TaxID=3869; Lupinus.
OH NCBI_TaxID=145753; Malva parviflora (Little mallow) (Cheeseweed mallow).
OH NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH NCBI_TaxID=23113; Philadelphus.
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH NCBI_TaxID=157662; Teramnus repens.
OH NCBI_TaxID=60916; Trifolium incarnatum (Crimson clover).
OH NCBI_TaxID=85293; Viburnum opulus (High-bush cranberry).
OH NCBI_TaxID=3916; Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OH NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=18640652; DOI=10.1016/0042-6822(86)90468-x;
RA Langereis K., Mugnier M.-A., Cornelissen B.J.C., Pinck L., Bol J.F.;
RT "Variable repeats and poly(A)-stretches in the leader sequence of alfalfa
RT mosaic virus RNA 3.";
RL Virology 154:409-414(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7433090; DOI=10.1093/nar/8.10.2213;
RA Brederode F.T., Koper-Zwarthoff E.C., Bol J.F.;
RT "Complete nucleotide sequence of alfalfa mosaic virus RNA 4.";
RL Nucleic Acids Res. 8:2213-2223(1980).
RN [3]
RP PROTEIN SEQUENCE, AND ACETYLATION AT SER-2.
RX PubMed=836394; DOI=10.1111/j.1432-1033.1977.tb11225.x;
RA van Beynum G.M.A., de Graaf J.M., Castel A., Kraal B., Bosch L.;
RT "Structural studies on the coat protein of alfalfa mosaic virus. The
RT complete primary structure.";
RL Eur. J. Biochem. 72:63-78(1977).
RN [4]
RP MUTAGENESIS.
RX PubMed=2014643; DOI=10.1016/0042-6822(91)90902-n;
RA Neeleman L., der Kuyl A.C., Bol J.F.;
RT "Role of alfalfa mosaic virus coat protein gene in symptom formation.";
RL Virology 181:687-693(1991).
RN [5]
RP FUNCTION.
RX PubMed=11717411; DOI=10.1073/pnas.251542798;
RA Neeleman L., Olsthoorn R.C., Linthorst H.J., Bol J.F.;
RT "Translation of a nonpolyadenylated viral RNA is enhanced by binding of
RT viral coat protein or polyadenylation of the RNA.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:14286-14291(2001).
RN [6]
RP INTERACTION WITH ARABIDOPSIS ALKBH9B.
RX PubMed=28923956; DOI=10.1073/pnas.1703139114;
RA Martinez-Perez M., Aparicio F., Lopez-Gresa M.P., Belles J.M.,
RA Sanchez-Navarro J.A., Pallas V.;
RT "Arabidopsis m6A demethylase activity modulates viral infection of a plant
RT virus and the m6A abundance in its genomic RNAs.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:10755-10760(2017).
CC -!- FUNCTION: Capsid protein. Binds to the to the 3' end of the
CC nonpolyadenylated viral RNA and is involved in viral RNA translation
CC initiation. Probably binds RNA and plays a role in packaging.
CC {ECO:0000269|PubMed:11717411}.
CC -!- SUBUNIT: Interacts with Arabidopsis ALKBH9B.
CC {ECO:0000269|PubMed:28923956}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the alphamovirus/ilarvirus capsid protein
CC family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Virus Particle ExploreR db; Note=Icosahedral capsid
CC structure;
CC URL="https://viperdb.scripps.edu/Info_Page.php?VDB=amv";
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DR EMBL; L00162; AAA46297.1; -; Genomic_RNA.
DR EMBL; V00048; CAA23418.1; -; Genomic_RNA.
DR EMBL; K03542; AAA46296.1; -; Genomic_RNA.
DR EMBL; M59241; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR SMR; P03591; -.
DR iPTMnet; P03591; -.
DR Proteomes; UP000000358; Genome.
DR GO; GO:0039617; C:T=3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006413; P:translational initiation; IEA:InterPro.
DR InterPro; IPR002681; Coat_Ilarvirus.
DR Pfam; PF01787; Ilar_coat; 1.
PE 1: Evidence at protein level;
KW Acetylation; Capsid protein; Direct protein sequencing;
KW Host-virus interaction; Reference proteome; Ribonucleoprotein; RNA-binding;
KW T=3 icosahedral capsid protein; Viral nucleoprotein; Virion.
FT INIT_MET 1
FT /note="Removed; by host"
FT CHAIN 2..221
FT /note="Capsid protein"
FT /id="PRO_0000083189"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine; by host"
FT /evidence="ECO:0000269|PubMed:836394"
FT MUTAGEN 29
FT /note="Q->R: Symptoms induced change from chlorosis to
FT necrosis."
FT /evidence="ECO:0000269|PubMed:2014643"
FT CONFLICT 216
FT /note="D -> N (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24370 MW; B6FC96713A0B73D7 CRC64;
MSSSQKKAGG KAGKPTKRSQ NYAALRKAQL PKPPALKVPV VKPTNTILPQ TGCVWQSLGT
PLSLSSFNGL GARFLYSFLK DFVGPRILEE DLIYRMVFSI TPSHAGTFCL TDDVTTEDGR
AVAHGNPMQE FPHGAFHANE KFGFELVFTA PTHAGMQNQN FKHSYAVALC LDFDAQPEGS
KNPSFRFNEV WVERKAFPRA GPLRSLITVG LFDEADDLDR H
