ID   Y1248_HAEIN             Reviewed;         322 AA.
AC   P44136;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Putative nickel/cobalt efflux system HI_1248;
GN   OrderedLocusNames=HI_1248;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Efflux system for nickel and cobalt. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC22907.1; -; Genomic_DNA.
DR   PIR; A64023; A64023.
DR   RefSeq; NP_439404.1; NC_000907.1.
DR   RefSeq; WP_005694309.1; NC_000907.1.
DR   AlphaFoldDB; P44136; -.
DR   STRING; 71421.HI_1248; -.
DR   EnsemblBacteria; AAC22907; AAC22907; HI_1248.
DR   KEGG; hin:HI_1248; -.
DR   PATRIC; fig|71421.8.peg.1300; -.
DR   eggNOG; COG2215; Bacteria.
DR   HOGENOM; CLU_058605_0_0_6; -.
DR   OMA; GLLLCWR; -.
DR   PhylomeDB; P44136; -.
DR   BioCyc; HINF71421:G1GJ1-1279-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0046583; F:cation efflux transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0035784; P:nickel cation homeostasis; IBA:GO_Central.
DR   GO; GO:0032025; P:response to cobalt ion; IBA:GO_Central.
DR   GO; GO:0010045; P:response to nickel cation; IBA:GO_Central.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   Pfam; PF03824; NicO; 2.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalt; Cobalt transport; Ion transport; Membrane; Nickel;
KW   Nickel transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..322
FT                   /note="Putative nickel/cobalt efflux system HI_1248"
FT                   /id="PRO_0000194014"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   322 AA;  35669 MW;  EC1248D2F25A1F47 CRC64;
     MKKYKTGLVL LVIALALLVY FSPWFFLHIA SWQKEFNQLI SENLHQIQNN SIKAGTTLIF
     ASFVYGVLHA LGPGHGKFII ASYLSTHESQ LKQSTILSLL SSLMQGIVAI TATTLLVVVL
     NLSSRYFKLS QLWLERTALL LLVFLGCYWI WQGLRAYRKK AKLAIKSLNP LPLHEKSAVK
     NNRTFQPNTC SCGHQHLPSP TQTAQATNLK SQFLVILTIG MRPCSGAIFV LFLAYMLDLY
     SWGILAVLAM SFGTGLMLSA FAGIVRYARN TAIHLGHWYS SKNTKGKSES IVKLIAGGIM
     LFFALSLLYG TTISTGGSKI LF