CAPSD_BPFD
ID CAPSD_BPFD Reviewed; 73 AA.
AC P69539; P03617;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Capsid protein G8P;
DE AltName: Full=Coat protein B;
DE AltName: Full=Gene 8 protein;
DE Short=G8P;
DE AltName: Full=Major coat protein;
DE Flags: Precursor;
GN Name=VIII;
OS Enterobacteria phage fd (Bacteriophage fd).
OC Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC Tubulavirales; Inoviridae; Inovirus.
OX NCBI_TaxID=2847073;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=478 / Heidelberg;
RX PubMed=745987; DOI=10.1093/nar/5.12.4495;
RA Beck E., Sommer R., Auerswald E.A., Kurz C., Zink B., Osterburg G.,
RA Schaller H., Sugimoto K., Sugisaki H., Okamoto T., Takanami M.;
RT "Nucleotide sequence of bacteriophage fd DNA.";
RL Nucleic Acids Res. 5:4495-4503(1978).
RN [2]
RP PROTEIN SEQUENCE OF 24-73.
RC STRAIN=478 / Heidelberg;
RX PubMed=5349375;
RA Asbeck F., Beyreuther K., Kohler H., von Wettstein G., Braunitzer G.;
RT "Virus proteins. IV. Constitution of the coat protein of the fd phage.";
RL Hoppe-Seyler's Z. Physiol. Chem. 350:1047-1066(1969).
RN [3]
RP PROTEIN SEQUENCE OF 24-73, AND SEQUENCE REVISION.
RX PubMed=4449122; DOI=10.1016/0022-2836(74)90410-0;
RA Nakashima Y., Konigsberg W.;
RT "Reinvestigation of a region of the fd bacteriophage coat protein
RT sequence.";
RL J. Mol. Biol. 88:598-600(1974).
RN [4]
RP STRUCTURE BY NMR.
RX PubMed=9237913; DOI=10.1006/jmbi.1997.1114;
RA Almeida F.C., Opella S.J.;
RT "fd coat protein structure in membrane environments: structural dynamics of
RT the loop between the hydrophobic trans-membrane helix and the amphipathic
RT in-plane helix.";
RL J. Mol. Biol. 270:481-495(1997).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).
RX PubMed=8289247; DOI=10.1016/s0022-2836(05)80032-4;
RA Marvin D.A., Hale R.D., Nave C., Citterich M.H.;
RT "Molecular models and structural comparisons of native and mutant class I
RT filamentous bacteriophages Ff (fd, f1, M13), If1 and IKe.";
RL J. Mol. Biol. 235:260-286(1994).
CC -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC genomic DNA. The capsid displays a filamentous structure with a length
CC of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC take place at the host inner membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes. There are several thousands of this protein
CC in the phage capsid (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane; Single-pass
CC type I membrane protein. Note=prior to assembly, the major capsid
CC protein is found associated with the bacterial host inner membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; J02451; AAA32308.1; -; Genomic_DNA.
DR PIR; A04226; VCBPFD.
DR RefSeq; YP_009111302.1; NC_025824.1.
DR PDB; 1FDM; NMR; -; A=24-73.
DR PDB; 1IFD; Fiber; 4.00 A; A=24-73.
DR PDB; 1IFI; Fiber; 3.30 A; A=24-73.
DR PDB; 1IFJ; Fiber; 3.30 A; A=24-73.
DR PDB; 1MZT; NMR; -; A=24-73.
DR PDB; 2C0W; X-ray; 3.20 A; A=24-73.
DR PDB; 2C0X; NMR; -; A=24-73.
DR PDB; 2HI5; EM; 8.00 A; A=24-73.
DR PDBsum; 1FDM; -.
DR PDBsum; 1IFD; -.
DR PDBsum; 1IFI; -.
DR PDBsum; 1IFJ; -.
DR PDBsum; 1MZT; -.
DR PDBsum; 2C0W; -.
DR PDBsum; 2C0X; -.
DR PDBsum; 2HI5; -.
DR BMRB; P69539; -.
DR SMR; P69539; -.
DR GeneID; 22475003; -.
DR KEGG; vg:22475003; -.
DR EvolutionaryTrace; P69539; -.
DR Proteomes; UP000001836; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.20.5.80; -; 1.
DR InterPro; IPR008020; G8P.
DR InterPro; IPR023390; Phage_M13_G8P_capsid_dom_sf.
DR Pfam; PF19199; Phage_coatGP8; 1.
DR PIRSF; PIRSF004117; Phage_coat_B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Direct protein sequencing;
KW Helical capsid protein; Host membrane; Membrane; Signal; Transmembrane;
KW Transmembrane helix; Virion.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:4449122,
FT ECO:0000269|PubMed:5349375"
FT CHAIN 24..73
FT /note="Capsid protein G8P"
FT /id="PRO_0000003296"
FT TOPO_DOM 24..44
FT /note="Periplasmic"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 66..73
FT /note="Cytoplasmic"
FT HELIX 28..70
FT /evidence="ECO:0007829|PDB:2C0W"
SQ SEQUENCE 73 AA; 7626 MW; 53ACF68DB18E6A52 CRC64;
MKKSLVLKAS VAVATLVPML SFAAEGDDPA KAAFDSLQAS ATEYIGYAWA MVVVIVGATI
GIKLFKKFTS KAS
