Y1278_HAEIN
ID Y1278_HAEIN Reviewed; 220 AA.
AC Q57431; O05050;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Putative NAD(P)H nitroreductase;
DE EC=1.-.-.-;
GN OrderedLocusNames=HI_1278;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=10675023;
RX DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA Fountoulakis M.;
RT "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL Electrophoresis 21:411-429(2000).
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000305};
CC -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR EMBL; L42023; AAC22926.1; -; Genomic_DNA.
DR PIR; B64114; B64114.
DR RefSeq; NP_439431.1; NC_000907.1.
DR RefSeq; WP_005694521.1; NC_000907.1.
DR PDB; 7S1A; X-ray; 1.97 A; A/B=1-220.
DR PDB; 7T2Z; X-ray; 2.25 A; A/B=1-220.
DR PDB; 7T33; X-ray; 2.30 A; A/B=1-220.
DR PDBsum; 7S1A; -.
DR PDBsum; 7T2Z; -.
DR PDBsum; 7T33; -.
DR AlphaFoldDB; Q57431; -.
DR SMR; Q57431; -.
DR STRING; 71421.HI_1278; -.
DR EnsemblBacteria; AAC22926; AAC22926; HI_1278.
DR KEGG; hin:HI_1278; -.
DR PATRIC; fig|71421.8.peg.1329; -.
DR eggNOG; COG0778; Bacteria.
DR HOGENOM; CLU_070764_4_1_6; -.
DR OMA; FAAWDTY; -.
DR PhylomeDB; Q57431; -.
DR BioCyc; HINF71421:G1GJ1-1303-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0018545; F:NAD(P)H nitroreductase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0046256; P:2,4,6-trinitrotoluene catabolic process; IBA:GO_Central.
DR CDD; cd02149; NfsB-like; 1.
DR Gene3D; 3.40.109.10; -; 1.
DR InterPro; IPR033878; NfsB-like.
DR InterPro; IPR029479; Nitroreductase.
DR InterPro; IPR000415; Nitroreductase-like.
DR Pfam; PF00881; Nitroreductase; 1.
DR SUPFAM; SSF55469; SSF55469; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Flavoprotein; FMN; NAD; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..220
FT /note="Putative NAD(P)H nitroreductase"
FT /id="PRO_0000072715"
FT BINDING 155..160
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT HELIX 6..15
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 20..22
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 30..41
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 52..57
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 60..66
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 71..73
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 74..79
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 81..90
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 97..105
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 110..125
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 134..158
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 171..180
FT /evidence="ECO:0007829|PDB:7S1A"
FT TURN 186..188
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 189..198
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:7S1A"
FT HELIX 212..215
FT /evidence="ECO:0007829|PDB:7S1A"
FT STRAND 216..219
FT /evidence="ECO:0007829|PDB:7S1A"
SQ SEQUENCE 220 AA; 25189 MW; AB0D804601BC975E CRC64;
MTQLTREQVL ELFHQRSSTR YYDPTKKISD EDFECILECG RLSPSSVGSE PWKFLVIQNK
TLREKMKPFS WGMINQLDNC SHLVVILAKK NARYDSPFFV DVMARKGLNA EQQQAALTKY
KALQEEDMKL LENDRTLFDW CSKQTYIALA NMLTGASALG IDSCPIEGFH YDKMNECLAE
EGLFDPQEYA VSVAATFGYR SRDIAKKSRK GLDEVVKWVG
