CAPSD_BPIF1
ID CAPSD_BPIF1 Reviewed; 74 AA.
AC P03619;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Capsid protein G8P;
DE AltName: Full=Coat protein B;
DE AltName: Full=Gene 8 protein;
DE Short=G8P;
DE AltName: Full=Major coat protein;
DE Flags: Precursor;
GN Name=VIII;
OS Escherichia phage If1 (Bacteriophage If1).
OC Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC Tubulavirales; Inoviridae; Infulavirus.
OX NCBI_TaxID=10868;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hill D.F., Hughes G., McNaughton J.C., Stockwell P.A., Petersen G.B.;
RT "DNA sequence of the filamentous coliphage If1.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 24-74.
RX PubMed=7240173; DOI=10.1016/s0021-9258(19)69276-8;
RA Nakashima Y., Frangione B., Wiseman R.L., Konigsberg W.H.;
RT "Primary structure of the major coat protein of the filamentous bacterial
RT viruses, If1 and Ike.";
RL J. Biol. Chem. 256:5792-5797(1981).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS).
RX PubMed=8289247; DOI=10.1016/s0022-2836(05)80032-4;
RA Marvin D.A., Hale R.D., Nave C., Citterich M.H.;
RT "Molecular models and structural comparisons of native and mutant class I
RT filamentous bacteriophages Ff (fd, f1, M13), If1 and IKe.";
RL J. Mol. Biol. 235:260-286(1994).
CC -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC genomic DNA. The capsid displays a filamentous structure with a length
CC of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC take place at the host inner membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes. There are several thousands of this protein
CC in the phage capsid (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane; Single-pass
CC type I membrane protein. Note=prior to assembly, the major capsid
CC protein is found associated with the bacterial host inner membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; U02303; AAC62154.1; -; Genomic_DNA.
DR PIR; A04227; VCBPIF.
DR RefSeq; NP_047355.1; NC_001954.1.
DR PDB; 1IFK; Fiber; 5.00 A; A=24-74.
DR PDBsum; 1IFK; -.
DR SMR; P03619; -.
DR GeneID; 1261854; -.
DR KEGG; vg:1261854; -.
DR EvolutionaryTrace; P03619; -.
DR Proteomes; UP000001833; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.20.5.80; -; 1.
DR InterPro; IPR008020; G8P.
DR InterPro; IPR023390; Phage_M13_G8P_capsid_dom_sf.
DR Pfam; PF19199; Phage_coatGP8; 1.
DR PIRSF; PIRSF004117; Phage_coat_B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Direct protein sequencing;
KW Helical capsid protein; Host membrane; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Virion.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:7240173"
FT CHAIN 24..74
FT /note="Capsid protein G8P"
FT /id="PRO_0000003300"
FT TOPO_DOM 24..45
FT /note="Periplasmic"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..74
FT /note="Cytoplasmic"
SQ SEQUENCE 74 AA; 7554 MW; 1BB72EF91BF29659 CRC64;
MKKSVVAKII AGSTLVIGSS AFAADDATSQ AKAAFDSLTA QATEMSGYAW ALVVLVVGAT
VGIKLFKKFV SRAS
