CAPSD_BPIKE
ID CAPSD_BPIKE Reviewed; 82 AA.
AC P03620;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Capsid protein G8P;
DE AltName: Full=Coat protein B;
DE AltName: Full=Gene 8 protein;
DE Short=G8P;
DE AltName: Full=Major coat protein;
DE Flags: Precursor;
GN Name=VIII;
OS Salmonella phage IKe (Bacteriophage IKe).
OC Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC Tubulavirales; Inoviridae; Lineavirus.
OX NCBI_TaxID=10867;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3981635; DOI=10.1016/0022-2836(85)90322-5;
RA Peeters B.P.H., Peters R.M., Schoenmakers J.G.G., Konings R.N.H.;
RT "Nucleotide sequence and genetic organization of the genome of the N-
RT specific filamentous bacteriophage IKe. Comparison with the genome of the
RT F-specific filamentous phages M13, fd and f1.";
RL J. Mol. Biol. 181:27-39(1985).
RN [2]
RP PROTEIN SEQUENCE OF 30-82.
RX PubMed=7240173; DOI=10.1016/s0021-9258(19)69276-8;
RA Nakashima Y., Frangione B., Wiseman R.L., Konigsberg W.H.;
RT "Primary structure of the major coat protein of the filamentous bacterial
RT viruses, If1 and Ike.";
RL J. Biol. Chem. 256:5792-5797(1981).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS).
RX PubMed=8289247; DOI=10.1016/s0022-2836(05)80032-4;
RA Marvin D.A., Hale R.D., Nave C., Citterich M.H.;
RT "Molecular models and structural comparisons of native and mutant class I
RT filamentous bacteriophages Ff (fd, f1, M13), If1 and IKe.";
RL J. Mol. Biol. 235:260-286(1994).
CC -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC genomic DNA. The capsid displays a filamentous structure with a length
CC of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC take place at the host inner membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes. There are several thousands of this protein
CC in the phage capsid (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane; Single-pass
CC type I membrane protein. Note=prior to assembly, the major capsid
CC protein is found associated with the bacterial host inner membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; X02139; CAA26072.1; -; Genomic_DNA.
DR PIR; A92912; VCBPIK.
DR RefSeq; NP_040575.1; NC_002014.1.
DR PDB; 1IFL; Fiber; 5.00 A; A=30-82.
DR PDB; 6A7F; EM; 3.40 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d=30-82.
DR PDBsum; 1IFL; -.
DR PDBsum; 6A7F; -.
DR SMR; P03620; -.
DR GeneID; 1260888; -.
DR KEGG; vg:1260888; -.
DR EvolutionaryTrace; P03620; -.
DR Proteomes; UP000000372; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.20.5.80; -; 1.
DR InterPro; IPR008020; G8P.
DR InterPro; IPR023390; Phage_M13_G8P_capsid_dom_sf.
DR Pfam; PF19199; Phage_coatGP8; 1.
DR PIRSF; PIRSF004117; Phage_coat_B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Direct protein sequencing;
KW Helical capsid protein; Host membrane; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Virion.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:7240173"
FT CHAIN 30..82
FT /note="Capsid protein G8P"
FT /id="PRO_0000003301"
FT TOPO_DOM 30..54
FT /note="Periplasmic"
FT TRANSMEM 55..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..82
FT /note="Cytoplasmic"
FT HELIX 39..81
FT /evidence="ECO:0007829|PDB:6A7F"
SQ SEQUENCE 82 AA; 8570 MW; C8F24F55A053799A CRC64;
MRVLSTVLAA KNKIALGAAT MLVSAGSFAA EPNAATNYAT EAMDSLKTQA IDLISQTWPV
VTTVVVAGLV IRLFKKFSSK AV
