ID   Y1287_METMA             Reviewed;         626 AA.
AC   Q8PXD4;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=UPF0313 protein MM_1287 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=MM_1287;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE008384; AAM30983.1; -; Genomic_DNA.
DR   RefSeq; WP_011033234.1; NC_003901.1.
DR   AlphaFoldDB; Q8PXD4; -.
DR   STRING; 192952.MM_1287; -.
DR   PRIDE; Q8PXD4; -.
DR   EnsemblBacteria; AAM30983; AAM30983; MM_1287.
DR   GeneID; 44085452; -.
DR   GeneID; 66137283; -.
DR   KEGG; mma:MM_1287; -.
DR   PATRIC; fig|192952.21.peg.1496; -.
DR   eggNOG; arCOG04984; Archaea.
DR   HOGENOM; CLU_018288_2_0_2; -.
DR   OMA; TCMYYTG; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..626
FT                   /note="UPF0313 protein MM_1287"
FT                   /id="PRO_0000076405"
FT   DOMAIN          320..589
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          206..227
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         334
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         338
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         341
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   626 AA;  70604 MW;  2AF499F8FF06F636 CRC64;
     MSPEEVKARG WKELDVILVT GDAYVDHSSF GTAIIGRVLE DAGFRVGIIA QPRWENPEDF
     KKLGKPRLFF SVSAGNTDSM VSNLTPGLKP RNKDAYSPGG KTGLRPNRAA IIYSNRIKEA
     FPDMPIVLGG IEASMRRFAH YDYLSDKVRQ SILADAPADL VVYGMGELQI VEIAKRLQAG
     EDIKGIRDIS GTVWKMEVKA WKELKGKGKE KAGEQDESEN ATEEVAKDKS LDPAEFFKEY
     VEIPSFSEVS QDKAVFAKAF RTYFLEQNPV TGKGIVQPHP KTIIIQNKPM RPLTEAELDH
     VYELPYTGET HPSYTEPVPA LEMVKFSLTT HRGCFGGCSF CAITQHQGRM IASRSIESIL
     REAKKLTQKP DFKGIINGVG GPTANMYGME CRSWEKKGAC LDKACLYPRV CPALDTSHKK
     LLELMHRLRE LPGVRHVFTG YGVRYDLALE DEEYLEELCA HHISGQLRIA PEHFSGRVTD
     AMSKPGKEVY EKFADRFNAF NKKCGKEQYI VNYLMSGHPG CTLKDMIEMA EYVRDHGGYT
     EQVQDFTPTP MTVSTCMYYT GLDPFTGKKM YVARDKKEKA MQRALMHYRN PANYELVYEA
     LEKAGRLDLV GNAHKCLIRR KERQRK