Y129_BORBU
ID Y129_BORBU Reviewed; 249 AA.
AC O51155;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase BB_0129;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=BB_0129;
OS Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS (Borrelia burgdorferi).
OC Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX NCBI_TaxID=224326;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=9403685; DOI=10.1038/37551;
RA Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA Smith H.O., Venter J.C.;
RT "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL Nature 390:580-586(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE000783; AAC66508.1; -; Genomic_DNA.
DR PIR; A70116; A70116.
DR RefSeq; NP_212263.1; NC_001318.1.
DR RefSeq; WP_002556731.1; NC_001318.1.
DR AlphaFoldDB; O51155; -.
DR SMR; O51155; -.
DR STRING; 224326.BB_0129; -.
DR EnsemblBacteria; AAC66508; AAC66508; BB_0129.
DR KEGG; bbu:BB_0129; -.
DR PATRIC; fig|224326.49.peg.527; -.
DR HOGENOM; CLU_024979_1_2_12; -.
DR OMA; SMEFAPF; -.
DR Proteomes; UP000001807; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding.
FT CHAIN 1..249
FT /note="Uncharacterized RNA pseudouridine synthase BB_0129"
FT /id="PRO_0000100025"
FT DOMAIN 7..64
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 112
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 249 AA; 28949 MW; 418BC2C82FD28669 CRC64;
MIALKAPRVH VFLAEKGVGS RRFCEELIRK KLVRVNNTIA KLGDKVTLGD RIIYKKQIFV
FKDFQINNRI YLALNKPRNY LCSNFDVDGR KLAISLVQPL FKERVFSIGR LDFKSSGLLL
FTNDGKFAND IIHPRQKVER EYIIESKKDI DENLLISFKS GIKVKKEFFK LKSYEILNKN
SARLILDEGK NREIRKVFLS KNIFLKKIHR IRIGNINLDS LKEGQVKIVP LSKINSLKSR
LEKLNDNSN
