CAPSD_BPPF1
ID CAPSD_BPPF1 Reviewed; 82 AA.
AC P03621;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 2.
DT 02-JUN-2021, entry version 120.
DE RecName: Full=Capsid protein G8P;
DE AltName: Full=Coat protein B;
DE AltName: Full=Gene 8 protein;
DE Short=G8P;
DE AltName: Full=Major coat protein;
DE Flags: Precursor;
GN Name=VIII;
OS Pseudomonas phage Pf1 (Bacteriophage Pf1).
OC Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC Tubulavirales; Inoviridae; Primolicivirus.
OX NCBI_TaxID=2011081;
OH NCBI_TaxID=287; Pseudomonas aeruginosa.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25102-B1 / pf;
RX PubMed=2010913; DOI=10.1016/0022-2836(91)90717-k;
RA Hill D.F., Short N.J., Perham R.N., Petersen G.B.;
RT "DNA sequence of the filamentous bacteriophage Pf1.";
RL J. Mol. Biol. 218:349-364(1991).
RN [2]
RP PROTEIN SEQUENCE OF 37-82.
RX PubMed=1110754; DOI=10.1038/253068a0;
RA Nakashima Y., Wiseman R.L., Konigsberg W., Marvin D.A.;
RT "Primary structure and sidechain interactions of Pf1 filamentous bacterial
RT virus coat protein.";
RL Nature 253:68-71(1975).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX PubMed=8036516; DOI=10.1126/science.8036516;
RA Liu D.J., Day L.A.;
RT "Pf1 virus structure: helical coat protein and DNA with paraxial
RT phosphates.";
RL Science 265:671-674(1994).
RN [4]
RP STRUCTURE BY NMR.
RX PubMed=1925543; DOI=10.1126/science.1925543;
RA Nambudripad R., Stark W., Opella S.J., Makowski L.;
RT "Membrane-mediated assembly of filamentous bacteriophage Pf1 coat
RT protein.";
RL Science 252:1305-1308(1991).
CC -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC genomic DNA. The capsid displays a filamentous structure with a length
CC of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC take place at the host inner membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes. There are several thousands of this protein
CC in the phage capsid (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane; Single-pass
CC type I membrane protein. Note=prior to assembly, the major capsid
CC protein is found associated with the bacterial host inner membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; X52107; CAA36331.1; -; Genomic_DNA.
DR PIR; A04229; VCBPPF.
DR PIR; S15143; S15143.
DR PDB; 1IFM; Fiber; 3.30 A; A=37-82.
DR PDB; 1IFN; Fiber; 4.00 A; A=37-82.
DR PDB; 1PFI; Fiber; 3.00 A; A/B=37-82.
DR PDB; 1PJF; NMR; -; A=37-82.
DR PDB; 1QL1; Fiber; 3.10 A; A=37-82.
DR PDB; 1QL2; Fiber; 3.10 A; A/B/C=37-82.
DR PDB; 1ZN5; NMR; -; A=37-82.
DR PDB; 2IFM; Fiber; 3.30 A; A=37-82.
DR PDB; 2IFN; Fiber; 4.00 A; A=37-82.
DR PDB; 2KLV; NMR; -; A=37-82.
DR PDB; 2KSJ; Other; -; A=37-82.
DR PDB; 2XKM; Other; 3.30 A; A=37-82.
DR PDB; 3IFM; Fiber; 3.30 A; A=37-82.
DR PDB; 4IFM; Fiber; 3.30 A; A=37-82.
DR PDB; 6TUP; EM; 3.20 A; A=37-82.
DR PDB; 6TUQ; EM; 3.90 A; A=37-82.
DR PDBsum; 1IFM; -.
DR PDBsum; 1IFN; -.
DR PDBsum; 1PFI; -.
DR PDBsum; 1PJF; -.
DR PDBsum; 1QL1; -.
DR PDBsum; 1QL2; -.
DR PDBsum; 1ZN5; -.
DR PDBsum; 2IFM; -.
DR PDBsum; 2IFN; -.
DR PDBsum; 2KLV; -.
DR PDBsum; 2KSJ; -.
DR PDBsum; 2XKM; -.
DR PDBsum; 3IFM; -.
DR PDBsum; 4IFM; -.
DR PDBsum; 6TUP; -.
DR PDBsum; 6TUQ; -.
DR BMRB; P03621; -.
DR SMR; P03621; -.
DR EvolutionaryTrace; P03621; -.
DR Proteomes; UP000002121; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR008020; G8P.
DR Pfam; PF05356; Phage_Coat_B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Direct protein sequencing;
KW Helical capsid protein; Host membrane; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Virion.
FT SIGNAL 1..28
FT CHAIN 29..82
FT /note="Capsid protein G8P"
FT /id="PRO_0000003302"
FT TOPO_DOM 29..57
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT TOPO_DOM 79..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 45
FT /note="E -> Q (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 39..51
FT /evidence="ECO:0007829|PDB:1PFI"
FT HELIX 53..81
FT /evidence="ECO:0007829|PDB:1PFI"
SQ SEQUENCE 82 AA; 8377 MW; 25FD055DA4661504 CRC64;
MKAMKQRIAK FSPVASFRNL CIAGSVTAAT SLPAFAGVID TSAVESAITD GQGDMKAIGG
YIVGALVILA VAGLIYSMLR KA
