CAPSD_BPPF3
ID CAPSD_BPPF3 Reviewed; 44 AA.
AC P03623;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-JUN-2021, entry version 108.
DE RecName: Full=Capsid protein G8P;
DE AltName: Full=Coat protein B;
DE AltName: Full=Gene 8 protein;
DE Short=G8P;
DE AltName: Full=Major coat protein;
DE AltName: Full=Pf3 coat protein;
GN Name=VIII;
OS Pseudomonas phage Pf3 (Bacteriophage Pf3).
OC Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC Tubulavirales; Inoviridae; Tertilicivirus.
OX NCBI_TaxID=10872;
OH NCBI_TaxID=287; Pseudomonas aeruginosa.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=New York, and Nijmegen;
RX PubMed=3928901; DOI=10.1128/jvi.56.1.268-276.1985;
RA Luiten R.G.M., Putterman D.G., Schoenmakers J.G.G., Konings R.N.H.,
RA Day L.A.;
RT "Nucleotide sequence of the genome of Pf3, an IncP-1 plasmid-specific
RT filamentous bacteriophage of Pseudomonas aeruginosa.";
RL J. Virol. 56:268-276(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6422463; DOI=10.1073/pnas.81.3.699;
RA Putterman D.G., Casadevall A., Boyle P.D., Yang H.-L., Frangione B.,
RA Day L.A.;
RT "Major coat protein and single-stranded DNA-binding protein of filamentous
RT virus Pf3.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:699-703(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6316283; DOI=10.1093/nar/11.22.8073;
RA Luiten R.G.M., Schoenmakers J.G.G., Konings R.N.H.;
RT "The major coat protein gene of the filamentous Pseudomonas aeruginosa
RT phage Pf3: absence of an N-terminal leader signal sequence.";
RL Nucleic Acids Res. 11:8073-8085(1983).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
RX PubMed=9761681; DOI=10.1006/jmbi.1998.2081;
RA Welsh L.C., Symmons M.F., Sturtevant J.M., Marvin D.A., Perham R.N.;
RT "Structure of the capsid of Pf3 filamentous phage determined from X-ray
RT fibre diffraction data at 3.1-A resolution.";
RL J. Mol. Biol. 283:155-177(1998).
RN [5]
RP TOPOLOGY.
RX PubMed=10562542; DOI=10.1093/emboj/18.22.6299;
RA Kiefer D., Kuhn A.;
RT "Hydrophobic forces drive spontaneous membrane insertion of the
RT bacteriophage Pf3 coat protein without topological control.";
RL EMBO J. 18:6299-6306(1999).
RN [6]
RP MEMBRANE INSERTION DEPENDS ON YIDC.
RX PubMed=12707259; DOI=10.1074/jbc.m301008200;
RA Chen M., Xie K., Nouwen N., Driessen A.J., Dalbey R.E.;
RT "Conditional lethal mutations separate the M13 procoat and Pf3 coat
RT functions of YidC: different YidC structural requirements for membrane
RT protein insertion.";
RL J. Biol. Chem. 278:23295-23300(2003).
CC -!- FUNCTION: Self assembles to form a helical capsid wrapping up the viral
CC genomic DNA. The capsid displays a filamentous structure with a length
CC of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding
CC take place at the host inner membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimerizes. There are several thousand copies of this
CC protein in the phage capsid (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC P03623; P25714: yidC; Xeno; NbExp=5; IntAct=EBI-8482910, EBI-6400779;
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host cell inner membrane;
CC Single-pass membrane protein. Note=Insertion into the host inner
CC membrane requires YidC but not the Sec translocon. Virion assembly
CC occurs after insertion.
CC -!- SIMILARITY: Belongs to the inovirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; X00167; CAA24992.1; -; Genomic_DNA.
DR EMBL; K01435; AAA72243.1; -; Genomic_DNA.
DR EMBL; M11912; AAA88378.1; -; Genomic_DNA.
DR EMBL; M19377; AAA88387.1; -; Genomic_DNA.
DR PIR; A04231; VCBPP3.
DR RefSeq; NP_040652.1; NC_001418.1.
DR PDB; 1IFP; Fiber; 3.10 A; A=1-44.
DR PDBsum; 1IFP; -.
DR SMR; P03623; -.
DR DIP; DIP-17009N; -.
DR IntAct; P03623; 1.
DR MINT; P03623; -.
DR GeneID; 1260904; -.
DR KEGG; vg:1260904; -.
DR EvolutionaryTrace; P03623; -.
DR Proteomes; UP000001719; Genome.
DR Proteomes; UP000009090; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR008020; G8P.
DR Pfam; PF05356; Phage_Coat_B; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Helical capsid protein;
KW Host cell inner membrane; Host cell membrane; Host membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Virion.
FT CHAIN 1..44
FT /note="Capsid protein G8P"
FT /id="PRO_0000098207"
FT TOPO_DOM 1..18
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:10562542"
FT TRANSMEM 19..39
FT /note="Helical"
FT TOPO_DOM 40..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:10562542"
FT HELIX 2..43
FT /evidence="ECO:0007829|PDB:1IFP"
SQ SEQUENCE 44 AA; 4631 MW; 7534B6279983AF77 CRC64;
MQSVITDVTG QLTAVQADIT TIGGAIIVLA AVVLGIRWIK AQFF
