CAPSD_BPPH2
ID CAPSD_BPPH2 Reviewed; 448 AA.
AC P13849; B3VMP3;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 2.
DT 29-SEP-2021, entry version 64.
DE RecName: Full=Major capsid protein {ECO:0000303|PubMed:17098197};
DE AltName: Full=Gene product 8 {ECO:0000303|PubMed:17098197};
DE Short=gp8 {ECO:0000303|PubMed:17098197};
DE AltName: Full=Major head protein {ECO:0000305};
DE AltName: Full=Protein p8 {ECO:0000305};
GN Name=8 {ECO:0000312|EMBL:ACE96030.1};
OS Bacillus phage phi29 (Bacteriophage phi-29).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Salasmaviridae; Picovirinae; Salasvirus.
OX NCBI_TaxID=10756;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3803926; DOI=10.1016/0378-1119(86)90406-3;
RA Vlcek C., Paces V.;
RT "Nucleotide sequence of the late region of Bacillus phage phi 29 completes
RT the 19,285-bp sequence of phi 29 genome. Comparison with the homologous
RT sequence of phage PZA.";
RL Gene 46:215-225(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Villegas A.P., Lingohr E.J., Ceyssens P.-J., Kropinski A.M.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
RX PubMed=3763399; DOI=10.1093/nar/14.17.7129;
RA Innis C.A., Garvey K.J., Ito J.;
RT "Nucleotide sequence of phage phi 29 gene 7: structure of intergenic spacer
RT between the major early and late genes.";
RL Nucleic Acids Res. 14:7129-7129(1986).
RN [4]
RP STRUCTURE BY ELECTRON MICROSCOPY OF THE VIRION, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=9814712; DOI=10.1016/s0092-8674(00)81773-0;
RA Tao Y., Olson N.H., Xu W., Anderson D.L., Rossmann M.G., Baker T.S.;
RT "Assembly of a tailed bacterial virus and its genome release studied in
RT three dimensions.";
RL Cell 95:431-437(1998).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=11562162; DOI=10.1006/jsbi.2001.4375;
RA Peterson C., Simon M., Hodges J., Mertens P., Higgins L., Egelman E.,
RA Anderson D.;
RT "Composition and mass of the bacteriophage phi29 prohead and virion.";
RL J. Struct. Biol. 135:18-25(2001).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (7.9 ANGSTROMS) OF THE VIRION, AND
RP FUNCTION.
RX PubMed=15837419; DOI=10.1016/j.molcel.2005.03.013;
RA Morais M.C., Choi K.H., Koti J.S., Chipman P.R., Anderson D.L.,
RA Rossmann M.G.;
RT "Conservation of the capsid structure in tailed dsDNA bacteriophages: the
RT pseudoatomic structure of phi29.";
RL Mol. Cell 18:149-159(2005).
RN [7]
RP CRYO-ELECTRON MICROSCOPY (20 ANGSTROMS) OF THE VIRION, FUNCTION, AND
RP SUBUNIT.
RX PubMed=17098197; DOI=10.1016/j.str.2006.09.007;
RA Choi K.H., Morais M.C., Anderson D.L., Rossmann M.G.;
RT "Determinants of bacteriophage phi29 head morphology.";
RL Structure 14:1723-1727(2006).
CC -!- FUNCTION: Assembles to form a prolate capsid shell of about 54 nm in
CC length and 45 nm in width, with a T=3, Q=5 symmetry.
CC {ECO:0000269|PubMed:15837419, ECO:0000269|PubMed:17098197,
CC ECO:0000269|PubMed:9814712}.
CC -!- SUBUNIT: Homohexamer. Homopentamer. The prolate capsid is composed of
CC pentamers and hexamers of the capsid protein.
CC {ECO:0000269|PubMed:17098197}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:11562162,
CC ECO:0000269|PubMed:9814712}. Note=Present in about 235 copies in the
CC virion. {ECO:0000269|PubMed:11562162, ECO:0000269|PubMed:9814712}.
CC -!- SIMILARITY: Belongs to the phi29likevirus major capsid protein family.
CC {ECO:0000305}.
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DR EMBL; M14782; AAA32280.1; -; Genomic_DNA.
DR EMBL; X04386; CAA27975.1; -; Genomic_DNA.
DR EMBL; EU771092; ACE96030.1; -; Genomic_DNA.
DR PIR; B25816; WMBP89.
DR RefSeq; YP_002004536.1; NC_011048.1.
DR PDB; 6QVK; EM; 3.60 A; 1A/1B/1C/1D/1E/1F/1G/1H/1I/1J/1K/1L/1M/1N/1O/1P/1Q/1R/1S/1T/1U/1V/1W/1X/1Y/1Z/1a/1b/1c/1d=1-448.
DR PDB; 6QYD; EM; 3.20 A; 1A/1B/1C/1D/1E/1F/1G/1H/1I/1J/1K/1L/1M/1N/1O/1P/1Q/1R/1S/1T/1U/1V/1W/1X/1Y/1Z/1a/1b/1c/1d=1-448.
DR PDB; 6QZ0; EM; 3.20 A; 1A/1B/1C/1D/1E/1F/1G/1H/1I/1J/1K/1L/1M/1N/1O/1P/1Q/1R/1S/1T/1U/1V/1W/1X/1Y/1Z/1a/1b/1c/1d=1-448.
DR PDBsum; 6QVK; -.
DR PDBsum; 6QYD; -.
DR PDBsum; 6QZ0; -.
DR SMR; P13849; -.
DR GeneID; 6446510; -.
DR KEGG; vg:6446510; -.
DR Proteomes; UP000001207; Genome.
DR GO; GO:0039617; C:T=3 icosahedral viral capsid; IDA:UniProtKB.
DR GO; GO:0046729; C:viral procapsid; IDA:CACAO.
DR InterPro; IPR003343; Big_2.
DR Pfam; PF02368; Big_2; 1.
DR SMART; SM00635; BID_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Coiled coil; Late protein;
KW Reference proteome; T=3 icosahedral capsid protein; Virion.
FT CHAIN 1..448
FT /note="Major capsid protein"
FT /id="PRO_0000106575"
FT DOMAIN 348..427
FT /note="BIG2"
FT /evidence="ECO:0000255"
FT COILED 107..127
FT /evidence="ECO:0000255"
FT CONFLICT 377
FT /note="N -> D (in Ref. 2; ACE96030)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 49844 MW; AC11DE58FEEA29FD CRC64;
MRITFNDVKT SLGITESYDI VNAIRNSQGD NFKSYVPLAT ANNVAEVGAG ILINQTVQND
FITSLVDRIG LVVIRQVSLN NPLKKFKKGQ IPLGRTIEEI YTDITKEKQY DAEEAEQKVF
EREMPNVKTL FHERNRQGFY HQTIQDDSLK TAFVSWGNFE SFVSSIINAI YNSAEVDEYE
YMKLLVDNYY SKGLFTTVKI DEPTSSTGAL TEFVKKMRAT ARKLTLPQGS RDWNSMAVRT
RSYMEDLHLI IDADLEAELD VDVLAKAFNM NRTDFLGNVT VIDGFASTGL EAVLVDKDWF
MVYDNLHKME TVRNPRGLYW NYYYHVWQTL SVSRFANAVA FVSGDVPAVT QVIVSPNIAA
VKQGGQQQFT AYVRATNAKD HKVVWSVEGG STGTAITGDG LLSVSGNEDN QLTVKATVDI
GTEDKPKLVV GEAVVSIRPN NASGGAQA
