ID   Y1370_MYCLE             Reviewed;         256 AA.
AC   O05668; Q49886;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase ML1370;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=ML1370; ORFNames=MLCB1351.03c, u0247g;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-256.
RA   Smith D.R., Robison K.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC31751.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z95117; CAB08276.1; -; Genomic_DNA.
DR   EMBL; AL583921; CAC31751.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00021; AAA50926.1; -; Genomic_DNA.
DR   PIR; D87080; D87080.
DR   PIR; S72955; S72955.
DR   RefSeq; WP_010908299.1; NC_002677.1.
DR   AlphaFoldDB; O05668; -.
DR   SMR; O05668; -.
DR   STRING; 272631.ML1370; -.
DR   EnsemblBacteria; CAC31751; CAC31751; CAC31751.
DR   KEGG; mle:ML1370; -.
DR   Leproma; ML1370; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_2_11; -.
DR   OMA; HVPKTYE; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..256
FT                   /note="Uncharacterized RNA pseudouridine synthase ML1370"
FT                   /id="PRO_0000100028"
FT   DOMAIN          16..83
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        121
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  28114 MW;  686746EF490FB695 CRC64;
     MVEIHDSRMD RGAGAVRLQK ILSRAGIASR RAAEKLIIEG RVEVDGQLVR ELGTRVDPDV
     SVVRVDGVKV VVDDSLVYLA LNKPRGMYST MSDDRGRPCV GDLIERRVRG NKKLFHVGRL
     DADTEGLILL TNDGELAHRL MHPSHEVSKT YLATVKGAVP RGLGKKLSVG LELDDGPAHV
     DDFAVVDAIP GKTLVRLTLH EGRKRIVRRL LTAAGFPVEM LVRTDIGAVS LGDQRPGCLR
     ALLRDEIRQL YKEVGL