ID   Y1380_AGRFC             Reviewed;         377 AA.
AC   Q8UFL7;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2002, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Putative zinc metalloprotease Atu1380;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=Atu1380; ORFNames=AGR_C_2553;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE007869; AAK87172.2; -; Genomic_DNA.
DR   PIR; AD2746; AD2746.
DR   PIR; C97527; C97527.
DR   RefSeq; NP_354387.2; NC_003062.2.
DR   AlphaFoldDB; Q8UFL7; -.
DR   SMR; Q8UFL7; -.
DR   STRING; 176299.Atu1380; -.
DR   EnsemblBacteria; AAK87172; AAK87172; Atu1380.
DR   KEGG; atu:Atu1380; -.
DR   PATRIC; fig|176299.10.peg.1403; -.
DR   eggNOG; COG0750; Bacteria.
DR   HOGENOM; CLU_025778_1_0_5; -.
DR   OMA; QYMVGFG; -.
DR   PhylomeDB; Q8UFL7; -.
DR   BioCyc; AGRO:ATU1380-MON; -.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   CHAIN           1..377
FT                   /note="Putative zinc metalloprotease Atu1380"
FT                   /id="PRO_0000088425"
FT   TRANSMEM        118..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..321
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        351..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          129..202
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        30
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         29
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         33
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   377 AA;  40225 MW;  BA4E755868BB101C CRC64;
     MNTIMAATGF LTGYIVPFIL VLSLLVFVHE MGHYLVGRWC GIRSTAFSIG FGPELIGFTD
     KRGTRWKLSA IPLGGYVKFF GDEDAASKSD SSGLSHMSLE ERAQTLSGAK LWKRAATVAA
     GPIANFILAI LIFAVLFGIY GRMIADPVVA EVRENSAAAT AGVKPGDRLV AIDGEKVMTF
     EDVRRYVGIR PGTPITVTVE RAGEELKLPM VPTRTETTDQ FGNKLEMGII GIVTDQTSGN
     FRHIEYSPSE AVAEGVRETG HVITGTFNYI GNLVTGRMNA DQLGGPVRVA QASGQMATLG
     ISAVIQLAAV LSVSIGLLNL MPVPVLDGGH LVFYAIEAIR GRPLGAGAQE VAFRIGMMMI
     LGLMVFATWN DISSLIG