Y1380_METJA
ID Y1380_METJA Reviewed; 109 AA.
AC Q58775;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 2.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Putative RNase MJ1380;
DE EC=3.1.-.- {ECO:0000250|UniProtKB:Q8ECH6};
DE AltName: Full=Putative toxin MJ1380;
GN OrderedLocusNames=MJ1380;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Probable toxic component of a putative type VII toxin-
CC antitoxin (TA) system, probably an RNase. Probably neutralized by
CC cognate antitoxin MJ1379. Neutralization may be due to AMPylation by
CC antitoxin MJ1379. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- SUBUNIT: Homodimer, probably forms a complex with cognate antitoxin
CC MJ1379. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- PTM: Modified by cognate antitoxin MJ1379; probably at least 2
CC successive AMPylation events occur on Tyr-83.
CC {ECO:0000250|UniProtKB:A0A0B0QJR1}.
CC -!- SIMILARITY: Belongs to the HepT RNase toxin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB99390.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L77117; AAB99390.1; ALT_FRAME; Genomic_DNA.
DR PIR; C64472; C64472.
DR AlphaFoldDB; Q58775; -.
DR SMR; Q58775; -.
DR STRING; 243232.MJ_1380; -.
DR EnsemblBacteria; AAB99390; AAB99390; MJ_1380.
DR KEGG; mja:MJ_1380; -.
DR eggNOG; arCOG05024; Archaea.
DR HOGENOM; CLU_142825_4_0_2; -.
DR InParanoid; Q58775; -.
DR PhylomeDB; Q58775; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR Gene3D; 1.20.120.580; -; 1.
DR InterPro; IPR008201; HepT-like.
DR InterPro; IPR037038; HepT-like_sf.
DR Pfam; PF01934; DUF86; 1.
PE 3: Inferred from homology;
KW Hydrolase; Nuclease; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..109
FT /note="Putative RNase MJ1380"
FT /id="PRO_0000158264"
FT MOTIF 76..83
FT /note="RX(4)HXY motif"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 76
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 81
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT MOD_RES 83
FT /note="O-di-AMP-tyrosine"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
SQ SEQUENCE 109 AA; 13058 MW; CA967C92E5E41087 CRC64;
MSKRDVKAFL YDILESANDV IEFTKDIDYN EFINNKMIRY AVIRALEIIG EASRYINNDF
REKFPNVPWK EMVGLRNILI HKYFGIDYIL LWKIVKEDVP KIKKEVEIV
