ID   Y1394_BIFLO             Reviewed;         435 AA.
AC   Q8G4I8;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Uncharacterized RNA methyltransferase BL1394;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=BL1394;
OS   Bifidobacterium longum (strain NCC 2705).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=206672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCC 2705;
RX   PubMed=12381787; DOI=10.1073/pnas.212527599;
RA   Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G.,
RA   Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.;
RT   "The genome sequence of Bifidobacterium longum reflects its adaptation to
RT   the human gastrointestinal tract.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE014295; AAN25193.1; -; Genomic_DNA.
DR   RefSeq; NP_696557.1; NC_004307.2.
DR   AlphaFoldDB; Q8G4I8; -.
DR   SMR; Q8G4I8; -.
DR   STRING; 206672.BL1394; -.
DR   PRIDE; Q8G4I8; -.
DR   EnsemblBacteria; AAN25193; AAN25193; BL1394.
DR   KEGG; blo:BL1394; -.
DR   PATRIC; fig|206672.9.peg.252; -.
DR   HOGENOM; CLU_014689_7_0_11; -.
DR   OMA; FYAGDMK; -.
DR   PhylomeDB; Q8G4I8; -.
DR   Proteomes; UP000000439; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 2.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..435
FT                   /note="Uncharacterized RNA methyltransferase BL1394"
FT                   /id="PRO_0000161958"
FT   ACT_SITE        393
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         261
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         294
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         318
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         366
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   435 AA;  47590 MW;  CE5FA186886C2663 CRC64;
     MPNSNRAQPE RTMMQAEVRI ERYADQGRCV AHIDGRVVFV RFALPDELVR VELDEPHDRD
     DRFWTGEVVE VLEPSEDRVT PAWPLAGPLA MGGGVGGADL VHVSLPGQLK WKAITVSEQM
     SRLGHIDVAV PIERMPGDKA AGGLNWRTRI EMIADDNGMP SMRRRGTHNR VAIDTMPLAT
     RTLLDVAKRE HVWEGGFEPG SQIRLSVPEP RGEIVDTAAA DDNYAVLVDG ELRAGSQLLT
     EQVTINGTTF DYQVDANGFW QVHRQAPIAL GTHVINLVNG QLQSAADAVI WDLYSGSGLF
     TLPLATMTGE RTRMLSVEGA RVAVKNAQRN LRAMNLNDVD ARAGDVSRTL DHVPAHLAKP
     NVVVLDPPRA GARAKVCRQI AAAGASSVVY IACDPTSLAR DTATLIGEGY ELKDIRAFDI
     YPMTHHVETV ALFTR