ID   Y1411_RALSO             Reviewed;         462 AA.
AC   Q8XZI4;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Putative zinc metalloprotease RSc1411;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=RSc1411; ORFNames=RS05281;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AL646052; CAD15113.1; -; Genomic_DNA.
DR   RefSeq; WP_011001360.1; NC_003295.1.
DR   AlphaFoldDB; Q8XZI4; -.
DR   SMR; Q8XZI4; -.
DR   STRING; 267608.RSc1411; -.
DR   EnsemblBacteria; CAD15113; CAD15113; RSc1411.
DR   GeneID; 60500934; -.
DR   KEGG; rso:RSc1411; -.
DR   PATRIC; fig|267608.8.peg.1442; -.
DR   eggNOG; COG0750; Bacteria.
DR   HOGENOM; CLU_025778_0_1_4; -.
DR   OMA; QYMVGFG; -.
DR   Proteomes; UP000001436; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; SSF50156; 2.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   CHAIN           1..462
FT                   /note="Putative zinc metalloprotease RSc1411"
FT                   /id="PRO_0000088454"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        430..450
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          201..283
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         18
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         22
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   462 AA;  49614 MW;  3F2818F8B0C1121B CRC64;
     MLTVLAFVFA IAVLIVVHEL GHYSVARLCG VKVLRFSVGF GKVLFRRVGR GPDRTEWTLC
     AIPLGGYVKM LGESARDPER DPPIPPEDLP RTFDHQPVYK RFAIVAAGPV FNFLLAIALY
     ALLAWVGAQE PLPILGAPPP GSIAAQADLR AKDRVVAVGT DEEAPTPVRA WSDVRMRLYE
     AGIGGRDAIV QVRGADGAER TVRLRELPSA ARSPQVDVIE QVGLRLLGGP VTIAEVLPGS
     AGERAGLRRG DQIVRFAGQP ADQASDLIRW IRAMPEQNAS IDILRDGLPM TLPVRLGADA
     DSANPGGPKL GKLGAQLSQH VETELIRDEP VHALGHAMRE VWRTSMLSLK VLGKMIVGQA
     SLQNLSGPIT VADFAGKAAS LGWQSFVAFL ALISVSLGVL NLLPVPVLDG GHLLYYCVEF
     LTGKPVPESW QAVLQKIGIA CILLLTSLAL YNDLSRLFLA HG