ID   Y1425_MYCTO             Reviewed;         459 AA.
AC   P9WKC0; L0T9D0; O06833; P71694;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT1468;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT1468;
GN   OrderedLocusNames=MT1468;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK45733.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK45733.1; ALT_INIT; Genomic_DNA.
DR   PIR; D70914; D70914.
DR   RefSeq; WP_003898875.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKC0; -.
DR   SMR; P9WKC0; -.
DR   EnsemblBacteria; AAK45733; AAK45733; MT1468.
DR   KEGG; mtc:MT1468; -.
DR   PATRIC; fig|83331.31.peg.1577; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..459
FT                   /note="Putative diacyglycerol O-acyltransferase MT1468"
FT                   /id="PRO_0000427680"
FT   ACT_SITE        138
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  50063 MW;  F436D6B588562263 CRC64;
     MKRLSSVDAA FWSAETAGWH MHVGALAICD PSDAPEYSFQ RLRELIIERL PEIPQLRWRV
     TGAPLGLDRP WFVEDEELDI DFHIRRIGVP APGGRRELEE LVGRLMSYKL DRSRPLWELW
     VIEGVEGGRI ATLTKMHHAI VDGVSGAGLG EILLDITPEP RPPQQETVGF VGFQIPGLER
     RAIGALINVG IMTPFRIVRL LEQTVRQQIA ALGVAGKPAR YFEAPKTRFN APVSPHRRVT
     GTRVELARAK AVKDAFGVKL NDVVLALVAG AARQYLQKRD ELPAKPLIAQ IPVSTRSEET
     KADVGNQVSS MTASLATHIE DPAKRLAAIH ESTLSAKEMA KAPSAHQIMG LTETTPPGLL
     QLAARAYTAS GLSHNLAPIN LVVSNVPGPP FPLYMAGARL DSLVPLGPPV MDVALNITCF
     SYQDYLDFGL VTTPEVANDI DEMADAIEPA LAELERAAE