CAPSD_FCVF9
ID CAPSD_FCVF9 Reviewed; 671 AA.
AC P27406;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 02-DEC-2020, entry version 81.
DE RecName: Full=Capsid protein;
DE AltName: Full=Coat protein;
DE Short=CP;
DE AltName: Full=VP1;
DE Contains:
DE RecName: Full=Protein 40k;
DE Flags: Precursor;
GN Name=CPP76; ORFNames=ORF2;
OS Feline calicivirus (strain F9) (FCV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Caliciviridae; Vesivirus.
OX NCBI_TaxID=11981;
OH NCBI_TaxID=9681; Felidae (cat family).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1529544; DOI=10.1016/0042-6822(92)91231-i;
RA Carter M.J., Milton I.D., Meanger J., Bennett M., Gaskell R.M.,
RA Turner P.C.;
RT "The complete nucleotide sequence of a feline calicivirus.";
RL Virology 190:443-448(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1633955; DOI=10.1042/bst020026s;
RA Meanger J., Carter M.J., Gaskell R.M., Turner P.C.;
RT "Cloning and sequence determination of the feline calicivirus strain F9.";
RL Biochem. Soc. Trans. 20:26S-26S(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 125-131, AND
RP PROTEOLYTIC PROCESSING.
RX PubMed=1731695; DOI=10.1007/bf01317185;
RA Carter M.J., Milton I.D., Turner P.C., Meanger J., Bennett M.,
RA Gaskell R.M.;
RT "Identification and sequence determination of the capsid protein gene of
RT feline calicivirus.";
RL Arch. Virol. 122:223-235(1992).
RN [4]
RP CLEAVAGE BY HOST CASPASE.
RX PubMed=12692289; DOI=10.1099/vir.0.18840-0;
RA Al-Molawi N., Beardmore V.A., Carter M.J., Kass G.E., Roberts L.O.;
RT "Caspase-mediated cleavage of the feline calicivirus capsid protein.";
RL J. Gen. Virol. 84:1237-1244(2003).
RN [5]
RP VIRAL ENTRY.
RX PubMed=16840330; DOI=10.1128/jvi.02452-05;
RA Stuart A.D., Brown T.D.;
RT "Entry of feline calicivirus is dependent on clathrin-mediated endocytosis
RT and acidification in endosomes.";
RL J. Virol. 80:7500-7509(2006).
RN [6]
RP INTERACTION WITH HOST F11R.
RX PubMed=17913818; DOI=10.1128/jvi.01509-07;
RA Ossiboff R.J., Parker J.S.L.;
RT "Identification of regions and residues in feline junctional adhesion
RT molecule required for feline calicivirus binding and infection.";
RL J. Virol. 81:13608-13621(2007).
RN [7]
RP INTERACTION WITH ALPHA2,6-LINKED SIALIC ACID.
RX PubMed=17170450; DOI=10.1099/vir.0.82158-0;
RA Stuart A.D., Brown T.D.;
RT "Alpha2,6-linked sialic acid acts as a receptor for Feline calicivirus.";
RL J. Gen. Virol. 88:177-186(2007).
RN [8]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.75 ANGSTROMS) OF 1-671.
RX PubMed=30626974; DOI=10.1038/s41586-018-0852-1;
RA Conley M.J., McElwee M., Azmi L., Gabrielsen M., Byron O., Goodfellow I.G.,
RA Bhella D.;
RT "Calicivirus VP2 forms a portal-like assembly following receptor
RT engagement.";
RL Nature 565:377-381(2019).
CC -!- FUNCTION: Capsid protein self assembles to form an icosahedral capsid
CC with a T=3 symmetry, about 38 nm in diameter, and consisting of 180
CC capsid proteins. A smaller form of capsid with a diameter of 23 nm
CC might be capsid proteins assembled as icosahedron with T=1 symmetry.
CC The capsid encapsulate the genomic RNA and VP2 proteins. Attaches
CC virion to target cells by binding to feline junctional adhesion
CC molecule A (F11R) and/or to alpha-2,6-linked sialic acid. Once
CC attached, the virion is endocytosed. Acidification of the endosome
CC induces conformational change of capsid protein thereby injecting virus
CC genomic RNA into host cytoplasm.
CC -!- SUBUNIT: Homodimerizes, then multimerizes. May bind to VP3 and Vpg
CC proteins. Binds to alpha-2,6-linked sialic acid at surface of target
CC cells. Interacts with host F11R. {ECO:0000269|PubMed:17170450,
CC ECO:0000269|PubMed:17913818}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm.
CC -!- PTM: Cleaved by virus calcivirin to produce mature capsid protein.
CC -!- PTM: Cleaved by host caspase-2 and caspase-6 to generate protein p40.
CC -!- SIMILARITY: Belongs to the caliciviridae capsid protein family.
CC {ECO:0000305}.
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DR EMBL; M86379; AAA79327.1; -; Genomic_RNA.
DR EMBL; Z11536; CAA77636.1; -; Genomic_RNA.
DR PIR; B43382; VCWWF9.
DR PDB; 6GSI; EM; 3.75 A; A/B/C/D=1-671.
DR PDBsum; 6GSI; -.
DR SMR; P27406; -.
DR Proteomes; UP000008762; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0039617; C:T=3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR CDD; cd00205; rhv_like; 1.
DR Gene3D; 2.60.120.20; -; 1.
DR InterPro; IPR004005; Calicivirus_coat.
DR InterPro; IPR033703; Rhv-like.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF00915; Calici_coat; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Direct protein sequencing; Host cytoplasm;
KW T=3 icosahedral capsid protein; Virion.
FT PROPEP 1..124
FT /evidence="ECO:0000269|PubMed:1731695"
FT /id="PRO_0000036878"
FT CHAIN 125..671
FT /note="Capsid protein"
FT /id="PRO_0000036879"
FT CHAIN ?465..671
FT /note="Protein 40k"
FT /id="PRO_0000341981"
FT SITE 124..125
FT /note="Cleavage; by calicivirin"
FT /evidence="ECO:0000250"
SQ SEQUENCE 671 AA; 73441 MW; 33BEE86D8370D5E5 CRC64;
MCSTCANVLK YYDWDPHFKL VINPNNFLSV GFCSNPLMCC YPELLPEFGT VWDCDRSPLE
IYLESILGDD EWASTFDAVD PVVPPMHWGA AGKIFQPHPG VLMHHLIGKV AAGWDPDLPL
IRLEADDGSI TAPEQGTMVG GVIAEPSAQM STAADMATGK SVDSEWEAFF SFHTSVNWST
SETQGKILFK QSLGPLLNPY LEHLAKLYVA WSGSIEVRFS ISGSGVFGGK LAAIVVPPGV
DPVQSTSMLQ YPHVLFDARQ VEPVIFCLPD LRSTLYHLMS DTDTTSLVIM VYNDLINPYA
NDANSSGCIV TVETKPGPDF KFHLLKPPGS MLTHGSIPSD LIPKTSSLWI GNRYWSDITD
FVIRPFVFQA NRHFDFNQET AGWSTPRFRP ISVTITEQNG AKLGIGVATD YIVPGIPDGW
PDTTIPGELI PAGDYAITNG TGNDITTATG YDTADIIKNN TNFRGMYICG SLQRAWGDKK
ISNTAFITTA TLDGDNNNKI NPCNTIDQSK IVVFQDNHVG KKAQTSDDTL ALLGYTGIGE
QAIGSDRDRV VRISTLPETG ARGGNHPIFY KNSIKLGYVI RSIDVFNSQI LHTSRQLSLN
HYLLPPDSFA VYRIIDSNGS WFDIGIDSDG FSFVGVSGFG KLEFPLSASY MGIQLAKIRL
ASNIRSPMTK L
