ID   Y1461_MYCTO             Reviewed;         846 AA.
AC   P9WFP6; L0T8B9; O53152; P67125;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=UPF0051 protein MT1508;
DE   Contains:
DE     RecName: Full=Endonuclease PI-MtuHIIP;
DE              EC=3.1.-.-;
DE     AltName: Full=Mtu pps1 intein;
GN   OrderedLocusNames=MT1508;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the UPF0051 (ycf24) family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45772.1; -; Genomic_DNA.
DR   PIR; H70871; H70871.
DR   RefSeq; WP_003407484.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WFP6; -.
DR   SMR; P9WFP6; -.
DR   PRIDE; P9WFP6; -.
DR   EnsemblBacteria; AAK45772; AAK45772; MT1508.
DR   KEGG; mtc:MT1508; -.
DR   PATRIC; fig|83331.31.peg.1622; -.
DR   HOGENOM; CLU_007402_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR010231; SUF_FeS_clus_asmbl_SufB.
DR   InterPro; IPR000825; SUF_FeS_clus_asmbl_SufBD.
DR   InterPro; IPR037284; SUF_FeS_clus_asmbl_SufBD_sf.
DR   InterPro; IPR045595; SufBD_N.
DR   Pfam; PF01458; SUFBD; 1.
DR   Pfam; PF19295; SufBD_N; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF101960; SSF101960; 2.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01980; sufB; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   Autocatalytic cleavage; Endonuclease; Hydrolase; Intron homing; Nuclease;
KW   Protein splicing.
FT   CHAIN           1..252
FT                   /note="UPF0051 protein MT1508, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000428500"
FT   CHAIN           253..611
FT                   /note="Endonuclease PI-MtuHIIP"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000428501"
FT   CHAIN           612..846
FT                   /note="UPF0051 protein MT1508, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000428502"
FT   DOMAIN          388..528
FT                   /note="DOD-type homing endonuclease"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   846 AA;  94171 MW;  468CEEF979B02222 CRC64;
     MTLTPEASKS VAQPPTQAPL TQEEAIASLG RYGYGWADSD VAGANAQRGL SEAVVRDISA
     KKNEPDWMLQ SRLKALRIFD RKPIPKWGSN LDGIDFDNIK YFVRSTEKQA ASWDDLPEDI
     RNTYDRLGIP EAEKQRLVAG VAAQYESEVV YHQIREDLEA QGVIFLDTDT GLREHPDIFK
     EYFGTVIPAG DNKFSALNTA VWSGGSFIYV PPGVHVDIPL QAYFRINTEN MGQFERTLII
     ADEGSYVHYV EGCLPAGELI TTADGDLRPI ESIRVGDFVT GHDGRPHRVT AVQVRDLDGE
     LFTFTPMSPA NAFSVTAEHP LLAIPRDEVR VMRKERNGWK AEVNSTKLRS AEPRWIAAKD
     VAEGDFLIYP KPKPIPHRTV LPLEFARLAG YYLAEGHACL TNGCESLIFS FHSDEFEYVE
     DVRQACKSLY EKSGSVLIEE HKHSARVTVY TKAGYAAMRD NVGIGSSNKK LSDLLMRQDE
     TFLRELVDAY VNGDGNVTRR NGAVWKRVHT TSRLWAFQLQ SILARLGHYA TVELRRPGGP
     GVIMGRNVVR KDIYQVQWTE GGRGPKQARD CGDYFAVPIK KRAVREAHEP VYNLDVENPD
     SYLAYGFAVH NCTAPIYKSD SLHSAVVEII VKPHARVRYT TIQNWSNNVY NLVTKRARAE
     AGATMEWIDG NIGSKVTMKY PAVWMTGEHA KGEVLSVAFA GEDQHQDTGA KMLHLAPNTS
     SNIVSKSVAR GGGRTSYRGL VQVNKGAHGS RSSVKCDALL VDTVSRSDTY PYVDIREDDV
     TMGHEATVSK VSENQLFYLM SRGLTEDEAM AMVVRGFVEP IAKELPMEYA LELNRLIELQ
     MEGAVG