Y1464_AQUAE
ID Y1464_AQUAE Reviewed; 249 AA.
AC O67444;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase aq_1464;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=aq_1464;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE000657; AAC07410.1; -; Genomic_DNA.
DR PIR; B70427; B70427.
DR RefSeq; NP_214009.1; NC_000918.1.
DR RefSeq; WP_010880947.1; NC_000918.1.
DR AlphaFoldDB; O67444; -.
DR SMR; O67444; -.
DR STRING; 224324.aq_1464; -.
DR EnsemblBacteria; AAC07410; AAC07410; aq_1464.
DR KEGG; aae:aq_1464; -.
DR PATRIC; fig|224324.8.peg.1141; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_2_0; -.
DR InParanoid; O67444; -.
DR OMA; HVPKTYE; -.
DR OrthoDB; 1037615at2; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding.
FT CHAIN 1..249
FT /note="Uncharacterized RNA pseudouridine synthase aq_1464"
FT /id="PRO_0000100023"
FT DOMAIN 4..71
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 106
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 249 AA; 28518 MW; 29C7A2E7916FDE7C CRC64;
MMEVRINKFL SEAGVASRRK AEKLILEGRV KVNGEVVRSL GVKVNPEVDI VEVDGKPVKP
QRKRYIILNK PCCYLTQLGR SPDGRKTIEE LIKDIPERVF PVGRLDYNTE GLLILTNDGE
LANRILHPRY KLPKVYLALV EGKVDQKTLK RMKQGIELED GFAKPDNIRI VRYEGKNTLL
EITFHEGRKH LVKRFLGAFG HKVKRLKRIA IGPIKLGKLS PGKWRELNQG ELAQLFKAVG
LKYVPRKRR
