Y1496_MYCTU
ID Y1496_MYCTU Reviewed; 336 AA.
AC P9WPZ1; L0T711; P63577; P71777;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=Probable GTPase Rv1496;
DE EC=3.6.-.-;
GN OrderedLocusNames=Rv1496; ORFNames=MTCY277.18;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP PROTEIN SEQUENCE OF 2-21, AND SEQUENCE REVISION TO N-TERMINUS.
RC STRAIN=H37Rv;
RX PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT annotated encoding genes.";
RL Genomics 114:292-304(2022).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH GDP, PROBABLE
RP FUNCTION, AND SUBUNIT.
RG Seattle structural genomics center for infectious disease (SSGCID);
RT "Crystal structure of arginine/ornithine transport system ATPase from
RT Mycobacterium tuberculosis bound to GDP.";
RL Submitted (APR-2010) to the PDB data bank.
CC -!- FUNCTION: Probable GTPase. May also bind and hydrolyze ATP. May
CC function as chaperone (Probable). {ECO:0000305}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|Ref.4}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCP44257.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR EMBL; AL123456; CCP44257.1; ALT_INIT; Genomic_DNA.
DR PIR; C70712; C70712.
DR RefSeq; NP_216012.1; NC_000962.3.
DR RefSeq; WP_003407596.1; NZ_NVQJ01000004.1.
DR PDB; 3MD0; X-ray; 2.45 A; A=3-336.
DR PDB; 3P32; X-ray; 1.90 A; A=3-336.
DR PDB; 4GT1; X-ray; 2.00 A; A=3-336.
DR PDBsum; 3MD0; -.
DR PDBsum; 3P32; -.
DR PDBsum; 4GT1; -.
DR AlphaFoldDB; P9WPZ1; -.
DR SMR; P9WPZ1; -.
DR STRING; 83332.Rv1496; -.
DR PaxDb; P9WPZ1; -.
DR DNASU; 886496; -.
DR GeneID; 45425476; -.
DR GeneID; 886496; -.
DR KEGG; mtu:Rv1496; -.
DR TubercuList; Rv1496; -.
DR eggNOG; COG1703; Bacteria.
DR OMA; WMWERID; -.
DR PhylomeDB; P9WPZ1; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR005129; GTPase_ArgK.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR23408; PTHR23408; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00750; lao; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Chaperone; Direct protein sequencing;
KW GTP-binding; Hydrolase; Nucleotide-binding; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:34915127"
FT CHAIN 2..336
FT /note="Probable GTPase Rv1496"
FT /id="PRO_0000157817"
FT BINDING 67..75
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 209
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 245..247
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT HELIX 11..19
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 25..34
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 38..51
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 58..64
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 71..83
FT /evidence="ECO:0007829|PDB:3P32"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 89..94
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 113..116
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 132..148
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 153..158
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 164..169
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 173..182
FT /evidence="ECO:0007829|PDB:3P32"
FT TURN 186..189
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 195..197
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 199..204
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 208..210
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 211..228
FT /evidence="ECO:0007829|PDB:3P32"
FT STRAND 239..243
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 250..266
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 269..295
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 298..312
FT /evidence="ECO:0007829|PDB:3P32"
FT HELIX 318..332
FT /evidence="ECO:0007829|PDB:3P32"
SQ SEQUENCE 336 AA; 36459 MW; 8B4AACF079CE66C7 CRC64;
MAMMAASHDD DTVDGLATAV RGGDRAALPR AITLVESTRP DHREQAQQLL LRLLPDSGNA
HRVGITGVPG VGKSTAIEAL GMHLIERGHR VAVLAVDPSS TRTGGSILGD KTRMARLAVH
PNAYIRPSPT SGTLGGVTRA TRETVVLLEA AGFDVILIET VGVGQSEVAV ANMVDTFVLL
TLARTGDQLQ GIKKGVLELA DIVVVNKADG EHHKEARLAA RELSAAIRLI YPREALWRPP
VLTMSAVEGR GLAELWDTVE RHRQVLTGAG EFDARRRDQQ VDWTWQLVRD AVLDRVWSNP
TVRKVRSELE RRVRAGELTP ALAAQQILEI ANLTDR