CAPSD_HEVCH
ID CAPSD_HEVCH Reviewed; 660 AA.
AC Q81871;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 29-SEP-2021, entry version 74.
DE RecName: Full=Secreted protein ORF2;
DE AltName: Full=Protein ORF2;
DE Short=pORF2;
GN ORFNames=ORF2;
OS Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Hepelivirales; Hepeviridae; Orthohepevirus; Hepatitis E virus.
OX NCBI_TaxID=652674;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8346669; DOI=10.1016/0168-1702(93)90024-h;
RA Bi S.L., Purdy M.A., McCaustland K.A., Margolis H.S., Bradley D.W.;
RT "The sequence of hepatitis E virus isolated directly from a single source
RT during an outbreak in China.";
RL Virus Res. 28:233-247(1993).
RN [2]
RP FUNCTION (ISOFORM CAPSID PROTEIN ORF2).
RX PubMed=19812150; DOI=10.1128/jvi.00717-09;
RA Kalia M., Chandra V., Rahman S.A., Sehgal D., Jameel S.;
RT "Heparan sulfate proteoglycans are required for cellular binding of the
RT hepatitis E virus ORF2 capsid protein and for viral infection.";
RL J. Virol. 83:12714-12724(2009).
RN [3]
RP FUNCTION (ISOFORM CAPSID PROTEIN ORF2), AND INTERACTION WITH HOST ASGR1 AND
RP ASGR2 (ISOFORM CAPSID PROTEIN ORF2).
RX PubMed=27155063; DOI=10.1002/jmv.24570;
RA Zhang L., Tian Y., Wen Z., Zhang F., Qi Y., Huang W., Zhang H., Wang Y.;
RT "Asialoglycoprotein receptor facilitates infection of PLC/PRF/5 cells by
RT HEV through interaction with ORF2.";
RL J. Med. Virol. 88:2186-2195(2016).
RN [4]
RP INTERACTION WITH HOST TMEM134 (ISOFORM CAPSID PROTEIN ORF2), AND
RP SUBCELLULAR LOCATION (ISOFORM CAPSID PROTEIN ORF2).
RC STRAIN=W2-1 HEV;
RX PubMed=27899274; DOI=10.1016/j.virusres.2016.11.027;
RA Tian Y., Huang W., Yang J., Wen Z., Geng Y., Zhao C., Zhang H., Wang Y.;
RT "Systematic identification of hepatitis E virus ORF2 interactome reveals
RT that TMEM134 engages in ORF2-mediated NF-kappaB pathway.";
RL Virus Res. 228:102-108(2017).
RN [5]
RP FUNCTION (ISOFORM SECRETED PROTEIN ORF2), SUBUNIT (ISOFORM SECRETED PROTEIN
RP ORF2), GLYCOSYLATION AT ASN-137; ASN-310 AND ASN-562 (ISOFORM SECRETED
RP PROTEIN ORF2), AND SUBCELLULAR LOCATION (ISOFORM SECRETED PROTEIN ORF2).
RX PubMed=29669922; DOI=10.1073/pnas.1721345115;
RA Yin X., Ying D., Lhomme S., Tang Z., Walker C.M., Xia N., Zheng Z.,
RA Feng Z.;
RT "Origin, antigenicity, and function of a secreted form of ORF2 in hepatitis
RT E virus infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:4773-4778(2018).
CC -!- FUNCTION: [Isoform Secreted protein ORF2]: Plays a role in the
CC inhibition of host antibody-mediated neutralization without blocking
CC viral cell entry. {ECO:0000269|PubMed:29669922}.
CC -!- FUNCTION: [Isoform Capsid protein ORF2]: Forms an icosahedral capsid
CC with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60
CC copies linked to each other (By similarity). Binds to the 5' end of the
CC genomic RNA to mediate genome encapsidation (By similarity). Binds to
CC heparin surface proteoglycans (HSPGs) to mediate viral entry
CC (PubMed:19812150). Additionally, the interactions with host ASGR1 and
CC ASGR2 facilitate viral infection of hepatocytes (PubMed:27155063).
CC {ECO:0000250|UniProtKB:P29326, ECO:0000269|PubMed:19812150,
CC ECO:0000269|PubMed:27155063}.
CC -!- SUBUNIT: [Isoform Secreted protein ORF2]: Homodimers.
CC {ECO:0000269|PubMed:29669922}.
CC -!- SUBUNIT: [Isoform Capsid protein ORF2]: Self-assembles to form the
CC capsid. The capsid is dominated by dimers that define the 30
CC morphological units. Interacts with phosphorylated protein ORF3 (By
CC similarity). Interacts with host TMEM134 (PubMed:27899274). Interacts
CC with host ASGR1 and ASGR2; these interactions facilitate infection of
CC host hepatocytes (PubMed:27155063). {ECO:0000250|UniProtKB:Q68985,
CC ECO:0000269|PubMed:27155063, ECO:0000269|PubMed:27899274, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Isoform Secreted protein ORF2]: Secreted
CC {ECO:0000269|PubMed:29669922}.
CC -!- SUBCELLULAR LOCATION: [Isoform Capsid protein ORF2]: Virion. Host
CC cytoplasm. Host endoplasmic reticulum {ECO:0000269|PubMed:27899274}.
CC Host Golgi apparatus {ECO:0000269|PubMed:27899274}. Host cell surface
CC {ECO:0000250|UniProtKB:Q68985}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Secreted protein ORF2;
CC IsoId=Q81871-1; Sequence=Displayed;
CC Name=Capsid protein ORF2;
CC IsoId=Q81871-2; Sequence=VSP_059886;
CC -!- PTM: [Isoform Secreted protein ORF2]: Exists as a glycosylated dimer.
CC {ECO:0000269|PubMed:29669922}.
CC -!- SIMILARITY: Belongs to the hepevirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; M94177; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; L08816; AAA03191.1; -; Genomic_RNA.
DR RefSeq; NP_056788.1; NC_001434.1.
DR SMR; Q81871; -.
DR iPTMnet; Q81871; -.
DR PRIDE; Q81871; -.
DR DNASU; 1494410; -.
DR GeneID; 1494410; -.
DR KEGG; vg:1494410; -.
DR Proteomes; UP000006705; Genome.
DR Proteomes; UP000102625; Genome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0044228; C:host cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0039615; C:T=1 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 1.
DR InterPro; IPR004261; SP2.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF03014; SP2; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Capsid protein; Glycoprotein; Host cytoplasm;
KW Host endoplasmic reticulum; Host Golgi apparatus; Host-virus interaction;
KW Reference proteome; RNA-binding; Secreted; T=1 icosahedral capsid protein;
KW Viral attachment to host cell; Viral attachment to host entry receptor;
KW Virion; Virus entry into host cell.
FT CHAIN 1..660
FT /note="Secreted protein ORF2"
FT /id="PRO_0000445486"
FT REGION 18..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 368..394
FT /note="particle formation"
FT /evidence="ECO:0000250"
FT REGION 585..610
FT /note="Oligomerization"
FT /evidence="ECO:0000250"
FT CARBOHYD 137
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000305|PubMed:29669922"
FT CARBOHYD 310
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000305|PubMed:29669922"
FT CARBOHYD 562
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000305|PubMed:29669922"
FT VAR_SEQ 1..15
FT /note="Missing (in isoform Capsid protein ORF2)"
FT /id="VSP_059886"
SQ SEQUENCE 660 AA; 70980 MW; B58F23955FDD6614 CRC64;
MRPRPILLLL LMFLPMLPAP PPGQPSGRRR GRRSGGSGGG FWGDRADSQP FAIPYIHPTN
PFAPDVTAAA GAGPRVRQPA RPLGSAWRDQ AQRPAAASRR RPTTAGAAPL TAVAPAHDTP
PVPDVDSRGA ILRRQYNLST SPLTSSVATG TNLVLYAAPL SPLLPLQDGT NTHIMATEAS
NYAQYRVVRA TIRYRPLVPN AVGGYAISIS FWPQTTTTPT SVDMNSITST DVRILVQPGI
ASEHVIPSER LHYRNQGWRS VETSGVAEEE ATSGLVMLCI HGSLVNSYTN TPYTGALGLL
DFALELEFRN LTPGNTNTRV SRYSSTARHR LRRGADGTAE LTTTAATRFM KDLYFTSTNG
VGEIGRGIAL TLFNLADTLL GGLPTELISS AGGQLFYSRP VVSANGEPTV KLYTSVENAQ
QDKGIAIPHD IDLGESRVVI QDYDNQHEQD RPTPSPAPSR PFSVLRANDV LWLSLTAAEY
DQSTYGSSTG PVYVSDSVTL VNVATGAQAV ARSLDWTKVT LDGRPLSTTQ QYSKTFFVLP
LRGKLSFWEA GTTKAGYPYN YNTTASDQLL VENAAGHRVA ISTYTTSLGA GPVSISAVAV
LAPHSALALL EDTMDYPARA HTFDDFCPEC RPLGLQGCAF QSTVAELQRL KMKVGKTREL
