CAPSD_HEVMY
ID CAPSD_HEVMY Reviewed; 660 AA.
AC Q04611;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 29-SEP-2021, entry version 69.
DE RecName: Full=Secreted protein ORF2;
DE AltName: Full=Protein ORF2;
DE Short=pORF2;
GN ORFNames=ORF2;
OS Hepatitis E virus genotype 1 (isolate Human/Myanmar/HEVNE8L) (HEV-1).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Hepelivirales; Hepeviridae; Orthohepevirus; Hepatitis E virus.
OX NCBI_TaxID=31769;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8470371; DOI=10.1007/bf01702352;
RA Aye T.T., Uchida T., Ma M.Z., Iida F., Shikata T., Ichikawa M.,
RA Rikihisa T., Winn K.;
RT "Sequence and gene structure of the hepatitis E virus isolated from
RT Myanmar.";
RL Virus Genes 7:95-109(1993).
CC -!- FUNCTION: [Isoform Secreted protein ORF2]: Plays a role in the
CC inhibition of host antibody-mediated neutralization without blocking
CC viral cell entry. {ECO:0000250|UniProtKB:Q81871}.
CC -!- FUNCTION: [Isoform Capsid protein ORF2]: Forms an icosahedral capsid
CC with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60
CC copies linked to each other. Binds to the 5' end of the genomic RNA to
CC mediate genome encapsidation (By similarity). Binds to heparin surface
CC proteoglycans (HSPGs) to mediate viral entry. Additionally, the
CC interactions with host ASGR1 and ASGR2 facilitate viral infection of
CC hepatocytes (By similarity). {ECO:0000250|UniProtKB:P29326,
CC ECO:0000250|UniProtKB:Q81871}.
CC -!- SUBUNIT: [Isoform Secreted protein ORF2]: Homodimers.
CC {ECO:0000250|UniProtKB:Q68985}.
CC -!- SUBUNIT: [Isoform Capsid protein ORF2]: Self-assembles to form the
CC capsid. The capsid is dominated by dimers that define the 30
CC morphological units. Interacts with phosphorylated protein ORF3 (By
CC similarity). Interacts with host TMEM134. Interacts with host ASGR1 and
CC ASGR2; these interactions facilitate infection of host hepatocytes (By
CC similarity). {ECO:0000250|UniProtKB:Q81871}.
CC -!- SUBCELLULAR LOCATION: [Isoform Secreted protein ORF2]: Secreted
CC {ECO:0000250|UniProtKB:Q81871}.
CC -!- SUBCELLULAR LOCATION: [Isoform Capsid protein ORF2]: Virion
CC {ECO:0000250|UniProtKB:Q81871}. Host cytoplasm
CC {ECO:0000250|UniProtKB:Q81871}. Host endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q81871}. Host Golgi apparatus
CC {ECO:0000250|UniProtKB:Q81871}. Host cell surface
CC {ECO:0000250|UniProtKB:Q68985}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Secreted protein ORF2;
CC IsoId=Q04611-1; Sequence=Displayed;
CC Name=Capsid protein ORF2;
CC IsoId=Q04611-2; Sequence=VSP_059888;
CC -!- PTM: [Isoform Secreted protein ORF2]: Exists as a glycosylated dimer.
CC {ECO:0000250|UniProtKB:Q81871}.
CC -!- SIMILARITY: Belongs to the hepevirus capsid protein family.
CC {ECO:0000305}.
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DR EMBL; D10330; BAA01174.1; -; Genomic_RNA.
DR SMR; Q04611; -.
DR Proteomes; UP000007246; Genome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0044228; C:host cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0039615; C:T=1 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 1.
DR InterPro; IPR004261; SP2.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF03014; SP2; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Capsid protein; Glycoprotein; Host cytoplasm;
KW Host endoplasmic reticulum; Host Golgi apparatus; Host-virus interaction;
KW RNA-binding; Secreted; T=1 icosahedral capsid protein;
KW Viral attachment to host cell; Viral attachment to host entry receptor;
KW Virion; Virus entry into host cell.
FT CHAIN 1..660
FT /note="Secreted protein ORF2"
FT /id="PRO_0000445488"
FT REGION 18..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 368..394
FT /note="particle formation"
FT /evidence="ECO:0000250"
FT REGION 585..610
FT /note="Oligomerization"
FT /evidence="ECO:0000250"
FT CARBOHYD 137
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000250|UniProtKB:Q81871"
FT CARBOHYD 310
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000250|UniProtKB:Q81871"
FT CARBOHYD 562
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000250|UniProtKB:Q81871"
FT VAR_SEQ 1..15
FT /note="Missing (in isoform Capsid protein ORF2)"
FT /id="VSP_059888"
SQ SEQUENCE 660 AA; 70998 MW; 3A82A4EA255C6253 CRC64;
MRPRPILLLL LMFLPMLPAP PPGQPSGRRR GRRSGGSGGG FWGDRVDSQP FAIPYIHPTN
PFAPDVTAAA GAGPRVRQPA RPLGSAWRDQ AQRPAVASRR RPTTAGAAPL TAVAPAHDTP
PVPDVDSRAA ILRRQYNLST SPLTSSVATG TNLVLYAAPL SPLLPLQDGT NTHIMATEAS
NYAQYRVVRA TIRYRPLVPN AVGGYAISIS FWPQTTTTPT SVDMNSITST DVRILVQPGI
ASELVIPSER LHYRNQGWRS VETSGVAEEE ATSGLVMLCI HGSPVNSYTN TPYTGALGLL
DFALELEFRN LTPGNTNTRV SRYSSTARHR LRRGADGTAE LTTTAATRFM KDLYFTSTNG
VGEIGRGIAL TLFNLADTLL GGLPTELISS AGGQLFYSRP VVSAHGEPTV KLYTSVENAQ
QDKGIAIPHD IDLGESRVVI QDYDNQHEQD RPTPSPAPSR PFSVLRANDV LWLSLTAAEY
DQSTYGSSTA PVYVSDSVTL VNVATGAQAV ARSLDWTKVT LDGRPLSTIQ QYPKTFFVLP
LRGKLSFWEA GTTKAGYPYN YNTTASDQLL VENAAGHRVA ISTYTTSLGA GPVSISAVAV
LAPHSALALL EDTLDYPACA HTFDDFCPEC RPLGLQGCAF QSTVAELQRL KMKVGKTREL
