ID   Y1533_MYCTU             Reviewed;         375 AA.
AC   O06179; L0T746;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Putative monooxygenase Rv1533;
DE            EC=1.13.12.-;
GN   OrderedLocusNames=Rv1533;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000250|UniProtKB:Q9HWH9};
CC       Note=Binds 1 FMN per subunit. {ECO:0000250|UniProtKB:Q9HWH9};
CC   -!- SIMILARITY: Belongs to the nitronate monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP44297.1; -; Genomic_DNA.
DR   PIR; B70500; B70500.
DR   RefSeq; NP_216049.1; NC_000962.3.
DR   RefSeq; WP_003898921.1; NZ_NVQJ01000004.1.
DR   AlphaFoldDB; O06179; -.
DR   SMR; O06179; -.
DR   STRING; 83332.Rv1533; -.
DR   PaxDb; O06179; -.
DR   DNASU; 886424; -.
DR   GeneID; 886424; -.
DR   KEGG; mtu:Rv1533; -.
DR   TubercuList; Rv1533; -.
DR   eggNOG; COG2070; Bacteria.
DR   InParanoid; O06179; -.
DR   OMA; PKYVGAE; -.
DR   PhylomeDB; O06179; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004314; F:[acyl-carrier-protein] S-malonyltransferase activity; IBA:GO_Central.
DR   GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR   CDD; cd04730; NPD_like; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR004136; NMO.
DR   Pfam; PF03060; NMO; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..375
FT                   /note="Putative monooxygenase Rv1533"
FT                   /id="PRO_0000393572"
FT   BINDING         190
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HWH9"
FT   BINDING         195
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HWH9"
FT   BINDING         224
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HWH9"
FT   BINDING         243..246
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HWH9"
SQ   SEQUENCE   375 AA;  39620 MW;  783C2D558D523010 CRC64;
     MRTRVAELLG AEFPICAFSH CRDVVAAVSN AGGFGILGAV AHSPKRLESE LTWIEEHTGG
     KPYGVDVLLP PKYIGAEQGG IDAQQARELI PEGHRTFVDD LLVRYGIPAV TDRQRSSSAG
     GLHISPKGYQ PLLDVAFAHD IRLIASALGP PPPDLVERAH NHDVLVAALA GTAQHARRHA
     AAGVDLIVAQ GTEAGGHTGE VATMVLVPEV VDAVSPTPVL AAGGIARGRQ IAAALALGAE
     GVWCGSVWLT TEEAETPPVV KDKFLAATSS DTVRSRSLTG KPARMLRTAW TDEWDRPDSP
     DPLGMPLQSA LVSDPQLRIN QAAGQPGAKA RELATYFVGQ VVGSLDRVRS ARSVVLDMVE
     EFIDTVGQLQ GLVQR