Y1534_ARATH
ID Y1534_ARATH Reviewed; 953 AA.
AC C0LGG7; Q9MAG1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Probable LRR receptor-like serine/threonine-protein kinase At1g53420;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At1g53420; ORFNames=F12M16.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=20064227; DOI=10.1186/1471-2164-11-19;
RA Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.;
RT "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-
RT like protein kinase genes in Arabidopsis thaliana.";
RL BMC Genomics 11:19-19(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=C0LGG7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=C0LGG7-2; Sequence=VSP_038280;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF69542.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC008007; AAF69542.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32938.1; -; Genomic_DNA.
DR EMBL; FJ708656; ACN59252.1; -; mRNA.
DR PIR; A96574; A96574.
DR RefSeq; NP_175747.2; NM_104220.2. [C0LGG7-1]
DR AlphaFoldDB; C0LGG7; -.
DR SMR; C0LGG7; -.
DR BioGRID; 27002; 11.
DR IntAct; C0LGG7; 11.
DR STRING; 3702.AT1G53420.1; -.
DR PaxDb; C0LGG7; -.
DR PRIDE; C0LGG7; -.
DR ProteomicsDB; 242475; -. [C0LGG7-1]
DR EnsemblPlants; AT1G53420.1; AT1G53420.1; AT1G53420. [C0LGG7-1]
DR GeneID; 841777; -.
DR Gramene; AT1G53420.1; AT1G53420.1; AT1G53420. [C0LGG7-1]
DR KEGG; ath:AT1G53420; -.
DR Araport; AT1G53420; -.
DR TAIR; locus:2009630; AT1G53420.
DR eggNOG; ENOG502QVI9; Eukaryota.
DR HOGENOM; CLU_000288_114_2_1; -.
DR InParanoid; C0LGG7; -.
DR OMA; GDEMSIN; -.
DR OrthoDB; 130579at2759; -.
DR PhylomeDB; C0LGG7; -.
DR PRO; PR:C0LGG7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; C0LGG7; baseline and differential.
DR Genevisible; C0LGG7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR021720; Malectin_dom.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF11721; Malectin; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Glycoprotein; Kinase;
KW Leucine-rich repeat; Membrane; Nucleotide-binding; Phosphoprotein;
KW Receptor; Reference proteome; Repeat; Serine/threonine-protein kinase;
KW Signal; Transferase; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..953
FT /note="Probable LRR receptor-like serine/threonine-protein
FT kinase At1g53420"
FT /id="PRO_0000387533"
FT TOPO_DOM 23..566
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 567..587
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 588..953
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 63..86
FT /note="LRR 1"
FT REPEAT 88..110
FT /note="LRR 2"
FT REPEAT 111..132
FT /note="LRR 3"
FT REPEAT 135..158
FT /note="LRR 4"
FT REPEAT 159..182
FT /note="LRR 5"
FT REPEAT 183..205
FT /note="LRR 6"
FT DOMAIN 624..901
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 750
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 630..638
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 652
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 697
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 783
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 784
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 789
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 797
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 265
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 315
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 378
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 423
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..255
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:20064227"
FT /id="VSP_038280"
SQ SEQUENCE 953 AA; 107490 MW; 9E9F961CB1D1DA90 CRC64;
MSLNRFLFTS FSFFLFFIVH FASSATLPTQ EGEAFKVVLT TLKKTNIDLN VDPCEVSSTG
NEWSTISRNL KRENLQGSLP KELVGLPLLQ EIDLSRNYLN GSIPPEWGVL PLVNIWLLGN
RLTGPIPKEF GNITTLTSLV LEANQLSGEL PLELGNLPNI QQMILSSNNF NGEIPSTFAK
LTTLRDFRVS DNQLSGTIPD FIQKWTKLER LFIQASGLVG PIPIAIASLV ELKDLRISDL
NGPESPFPQL RNIKKMETLI LRNCNLTGDL PDYLGKITSF KFLDLSFNKL SGAIPNTYIN
LRDGGYIYFT GNMLNGSVPD WMVNKGYKID LSYNNFSVDP TNAVCKYNNV LSCMRNYQCP
KTFNALHINC GGDEMSINGT IYESDKYDRL ESWYESRNGW FSNNVGVFVD DKHVPERVTI
ESNSSELNVV DFGLYTQARI SAISLTYYAL CLENGNYNVN LHFAEIMFNG NNNYQSLGRR
FFDIYIQRKL EVKDFNIAKE AKDVGNVVIK TFPVEIKDGK LEIRLYWAGR GTTVIPKERV
YGPLISAISV DSSVNPSPRN GMSTGTLHTL VVILSIFIVF LVFGTLWKKG YLRSKSQMEK
DFKSLELMIA SFSLRQIKIA TNNFDSANRI GEGGFGPVYK GKLFDGTIIA VKQLSTGSKQ
GNREFLNEIG MISALHHPNL VKLYGCCVEG GQLLLVYEFV ENNSLARALF GPQETQLRLD
WPTRRKICIG VARGLAYLHE ESRLKIVHRD IKATNVLLDK QLNPKISDFG LAKLDEEDST
HISTRIAGTF GYMAPEYAMR GHLTDKADVY SFGIVALEIV HGRSNKIERS KNNTFYLIDW
VEVLREKNNL LELVDPRLGS EYNREEAMTM IQIAIMCTSS EPCERPSMSE VVKMLEGKKM
VEVEKLEEAS VHRETKRLEN MNTMKKYYEM IGQEISTSMS MIMSDRSESS ADH
