ID   Y1540_MYCTU             Reviewed;         308 AA.
AC   P9WHQ3; L0T9Q9; P0A5T2; Q10786;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase Rv1540;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=Rv1540; ORFNames=MTCY48.25c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP44304.1; -; Genomic_DNA.
DR   PIR; A70761; A70761.
DR   RefSeq; NP_216056.1; NC_000962.3.
DR   RefSeq; WP_003407723.1; NZ_NVQJ01000004.1.
DR   AlphaFoldDB; P9WHQ3; -.
DR   SMR; P9WHQ3; -.
DR   STRING; 83332.Rv1540; -.
DR   PaxDb; P9WHQ3; -.
DR   DNASU; 886404; -.
DR   GeneID; 45425523; -.
DR   GeneID; 886404; -.
DR   KEGG; mtu:Rv1540; -.
DR   TubercuList; Rv1540; -.
DR   eggNOG; COG0564; Bacteria.
DR   OMA; AHPSPGW; -.
DR   PhylomeDB; P9WHQ3; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..308
FT                   /note="Uncharacterized RNA pseudouridine synthase Rv1540"
FT                   /id="PRO_0000162743"
FT   DOMAIN          15..81
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   308 AA;  33017 MW;  9CE52148701D7C26 CRC64;
     MADRSMPVPD GLAGMRVDTG LARLLGLSRT AAAALAEEGA VELNGVPAGK SDRLVSGALL
     QVRLPEAPAP LQNTPIDIEG MTILYSDDDI VAVDKPAAVA AHASVGWTGP TVLGGLAAAG
     YRITTSGVHE RQGIVHRLDV GTSGVMVVAI SERAYTVLKR AFKYRTVDKR YHALVQGHPD
     PSSGTIDAPI GRHRGHEWKF AITKNGRHSL THYDTLEAFV AASLLDVHLE TGRTHQIRVH
     FAALHHPCCG DLVYGADPKL AKRLGLDRQW LHARSLAFAH PADGRRVEIV SPYPADLQHA
     LKILRGEG