ID   Y1567_MYCBO             Reviewed;         308 AA.
AC   P0A5T3; A0A1R3XYM4; Q10786; X2BIN2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase Mb1567;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=BQ2027_MB1567;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIU00170.1; -; Genomic_DNA.
DR   RefSeq; NP_855219.1; NC_002945.3.
DR   RefSeq; WP_003407723.1; NC_002945.4.
DR   AlphaFoldDB; P0A5T3; -.
DR   SMR; P0A5T3; -.
DR   EnsemblBacteria; SIU00170; SIU00170; BQ2027_MB1567.
DR   GeneID; 45425523; -.
DR   PATRIC; fig|233413.5.peg.1713; -.
DR   OMA; AHPSPGW; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding.
FT   CHAIN           1..308
FT                   /note="Uncharacterized RNA pseudouridine synthase Mb1567"
FT                   /id="PRO_0000162744"
FT   DOMAIN          15..81
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   308 AA;  33017 MW;  9CE52148701D7C26 CRC64;
     MADRSMPVPD GLAGMRVDTG LARLLGLSRT AAAALAEEGA VELNGVPAGK SDRLVSGALL
     QVRLPEAPAP LQNTPIDIEG MTILYSDDDI VAVDKPAAVA AHASVGWTGP TVLGGLAAAG
     YRITTSGVHE RQGIVHRLDV GTSGVMVVAI SERAYTVLKR AFKYRTVDKR YHALVQGHPD
     PSSGTIDAPI GRHRGHEWKF AITKNGRHSL THYDTLEAFV AASLLDVHLE TGRTHQIRVH
     FAALHHPCCG DLVYGADPKL AKRLGLDRQW LHARSLAFAH PADGRRVEIV SPYPADLQHA
     LKILRGEG