Y1571_ARATH
ID Y1571_ARATH Reviewed; 1120 AA.
AC Q9LP24; C0LGF8; C0Z3K1; Q94B73;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Probable leucine-rich repeat receptor-like protein kinase At1g35710;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At1g35710; ORFNames=F14D7.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=20064227; DOI=10.1186/1471-2164-11-19;
RA Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.;
RT "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-
RT like protein kinase genes in Arabidopsis thaliana.";
RL BMC Genomics 11:19-19(2010).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-708 (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- INTERACTION:
CC Q9LP24-3; C0LGE0: At1g07650; NbExp=3; IntAct=EBI-20651291, EBI-16954597;
CC Q9LP24-3; Q9LT96: At5g49770; NbExp=3; IntAct=EBI-20651291, EBI-17123993;
CC Q9LP24-3; C0LGN2: LRR-RLK; NbExp=3; IntAct=EBI-20651291, EBI-20652801;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9LP24-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LP24-2; Sequence=VSP_038434;
CC Name=3;
CC IsoId=Q9LP24-3; Sequence=VSP_038435;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79881.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAK68748.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC021198; AAF79881.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31824.1; -; Genomic_DNA.
DR EMBL; AK319165; BAH57280.1; -; mRNA.
DR EMBL; FJ708647; ACN59243.1; -; mRNA.
DR EMBL; AY042808; AAK68748.1; ALT_INIT; mRNA.
DR PIR; B86479; B86479.
DR RefSeq; NP_174809.1; NM_103273.2. [Q9LP24-1]
DR AlphaFoldDB; Q9LP24; -.
DR SMR; Q9LP24; -.
DR BioGRID; 25707; 14.
DR IntAct; Q9LP24; 17.
DR STRING; 3702.AT1G35710.1; -.
DR PaxDb; Q9LP24; -.
DR PRIDE; Q9LP24; -.
DR ProteomicsDB; 242438; -. [Q9LP24-1]
DR EnsemblPlants; AT1G35710.1; AT1G35710.1; AT1G35710. [Q9LP24-1]
DR GeneID; 840475; -.
DR Gramene; AT1G35710.1; AT1G35710.1; AT1G35710. [Q9LP24-1]
DR KEGG; ath:AT1G35710; -.
DR Araport; AT1G35710; -.
DR TAIR; locus:2011339; AT1G35710.
DR eggNOG; ENOG502QQYD; Eukaryota.
DR HOGENOM; CLU_000288_22_1_1; -.
DR InParanoid; Q9LP24; -.
DR OMA; SICANTF; -.
DR OrthoDB; 179030at2759; -.
DR PhylomeDB; Q9LP24; -.
DR PRO; PR:Q9LP24; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LP24; baseline and differential.
DR Genevisible; Q9LP24; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 5.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR Pfam; PF13855; LRR_8; 4.
DR Pfam; PF08263; LRRNT_2; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00369; LRR_TYP; 13.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51450; LRR; 23.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Glycoprotein; Kinase;
KW Leucine-rich repeat; Membrane; Nucleotide-binding; Phosphoprotein;
KW Receptor; Reference proteome; Repeat; Serine/threonine-protein kinase;
KW Signal; Transferase; Transmembrane; Transmembrane helix.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..1120
FT /note="Probable leucine-rich repeat receptor-like protein
FT kinase At1g35710"
FT /id="PRO_0000389461"
FT TOPO_DOM 30..783
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 784..804
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 805..1120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 78..100
FT /note="LRR 1"
FT REPEAT 103..125
FT /note="LRR 2"
FT REPEAT 127..150
FT /note="LRR 3"
FT REPEAT 151..172
FT /note="LRR 4"
FT REPEAT 175..198
FT /note="LRR 5"
FT REPEAT 199..221
FT /note="LRR 6"
FT REPEAT 223..246
FT /note="LRR 7"
FT REPEAT 247..269
FT /note="LRR 8"
FT REPEAT 271..294
FT /note="LRR 9"
FT REPEAT 295..317
FT /note="LRR 10"
FT REPEAT 319..342
FT /note="LRR 11"
FT REPEAT 343..365
FT /note="LRR 12"
FT REPEAT 367..389
FT /note="LRR 13"
FT REPEAT 391..412
FT /note="LRR 14"
FT REPEAT 415..437
FT /note="LRR 15"
FT REPEAT 439..461
FT /note="LRR 16"
FT REPEAT 463..484
FT /note="LRR 17"
FT REPEAT 487..510
FT /note="LRR 18"
FT REPEAT 535..557
FT /note="LRR 19"
FT REPEAT 559..581
FT /note="LRR 20"
FT REPEAT 583..605
FT /note="LRR 21"
FT REPEAT 607..630
FT /note="LRR 22"
FT REPEAT 631..652
FT /note="LRR 23"
FT REPEAT 655..677
FT /note="LRR 24"
FT REPEAT 678..701
FT /note="LRR 25"
FT REPEAT 702..723
FT /note="LRR 26"
FT REPEAT 726..748
FT /note="LRR 27"
FT DOMAIN 851..1120
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 981
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10028"
FT BINDING 857..865
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 878
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 848
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O22476"
FT MOD_RES 929
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O22476"
FT MOD_RES 968
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:C0LGT6"
FT MOD_RES 1014
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9M0G7"
FT MOD_RES 1022
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:C0LGT6"
FT MOD_RES 1029
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9M0G7"
FT MOD_RES 1030
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9M0G7"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 295
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 460
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 473
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 490
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 569
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 580
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 604
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 607
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 641
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 660
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 712
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..412
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172,
FT ECO:0000303|PubMed:19423640"
FT /id="VSP_038434"
FT VAR_SEQ 825..1120
FT /note="ETGENMSIFSVDGKFKYQDIIESTNEFDPTHLIGTGGYSKVYRANLQDTIIA
FT VKRLHDTIDEEISKPVVKQEFLNEVKALTEIRHRNVVKLFGFCSHRRHTFLIYEYMEKG
FT SLNKLLANDEEAKRLTWTKRINVVKGVAHALSYMHHDRITPIVHRDISSGNILLDNDYT
FT AKISDFGTAKLLKTDSSNWSAVAGTYGYVAPEFAYTMKVTEKCDVYSFGVLILELIIGK
FT HPGDLVSSLSSSPGEALSLRSISDERVLEPRGQNREKLLKMVEMALLCLQANPESRPTM
FT LSISTTFS -> HRNRRIQQSLQSKPPRYNHSR (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:20064227"
FT /id="VSP_038435"
FT CONFLICT 531
FT /note="G -> W (in Ref. 3; BAH57280)"
FT /evidence="ECO:0000305"
FT CONFLICT 710
FT /note="H -> Y (in Ref. 4; ACN59243)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9LP24-2:644
FT /note="G -> A (in Ref. 5; AAK68748)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9LP24-2:655
FT /note="G -> A (in Ref. 5; AAK68748)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9LP24-2:670
FT /note="E -> A (in Ref. 5; AAK68748)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9LP24-2:677
FT /note="E -> Q (in Ref. 5; AAK68748)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1120 AA; 124104 MW; DF5A5A0BA67B1357 CRC64;
MGFAEKNLYD FRFLLFISII LSCSISASAT IAEANALLKW KSTFTNSSKL SSWVHDANTN
TSFSCTSWYG VSCNSRGSIE ELNLTNTGIE GTFQDFPFIS LSNLAYVDLS MNLLSGTIPP
QFGNLSKLIY FDLSTNHLTG EISPSLGNLK NLTVLYLHQN YLTSVIPSEL GNMESMTDLA
LSQNKLTGSI PSSLGNLKNL MVLYLYENYL TGVIPPELGN MESMTDLALS QNKLTGSIPS
TLGNLKNLMV LYLYENYLTG VIPPEIGNME SMTNLALSQN KLTGSIPSSL GNLKNLTLLS
LFQNYLTGGI PPKLGNIESM IDLELSNNKL TGSIPSSLGN LKNLTILYLY ENYLTGVIPP
ELGNMESMID LQLNNNKLTG SIPSSFGNLK NLTYLYLYLN YLTGVIPQEL GNMESMINLD
LSQNKLTGSV PDSFGNFTKL ESLYLRVNHL SGAIPPGVAN SSHLTTLILD TNNFTGFFPE
TVCKGRKLQN ISLDYNHLEG PIPKSLRDCK SLIRARFLGN KFTGDIFEAF GIYPDLNFID
FSHNKFHGEI SSNWEKSPKL GALIMSNNNI TGAIPTEIWN MTQLVELDLS TNNLFGELPE
AIGNLTNLSR LRLNGNQLSG RVPAGLSFLT NLESLDLSSN NFSSEIPQTF DSFLKLHDMN
LSRNKFDGSI PRLSKLTQLT QLDLSHNQLD GEIPSQLSSL QSLDKLDLSH NNLSGLIPTT
FEGMIALTNV DISNNKLEGP LPDTPTFRKA TADALEENIG LCSNIPKQRL KPCRELKKPK
KNGNLVVWIL VPILGVLVIL SICANTFTYC IRKRKLQNGR NTDPETGENM SIFSVDGKFK
YQDIIESTNE FDPTHLIGTG GYSKVYRANL QDTIIAVKRL HDTIDEEISK PVVKQEFLNE
VKALTEIRHR NVVKLFGFCS HRRHTFLIYE YMEKGSLNKL LANDEEAKRL TWTKRINVVK
GVAHALSYMH HDRITPIVHR DISSGNILLD NDYTAKISDF GTAKLLKTDS SNWSAVAGTY
GYVAPEFAYT MKVTEKCDVY SFGVLILELI IGKHPGDLVS SLSSSPGEAL SLRSISDERV
LEPRGQNREK LLKMVEMALL CLQANPESRP TMLSISTTFS
