ID   Y1606_LACJO             Reviewed;         459 AA.
AC   Q74IG2;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Uncharacterized RNA methyltransferase LJ_1606;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=LJ_1606;
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533;
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE017198; AAS09376.1; -; Genomic_DNA.
DR   RefSeq; WP_011162306.1; NC_005362.1.
DR   AlphaFoldDB; Q74IG2; -.
DR   SMR; Q74IG2; -.
DR   STRING; 257314.LJ_1606; -.
DR   EnsemblBacteria; AAS09376; AAS09376; LJ_1606.
DR   KEGG; ljo:LJ_1606; -.
DR   PATRIC; fig|257314.6.peg.1427; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_1_9; -.
DR   OMA; YCGVGGF; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..459
FT                   /note="Uncharacterized RNA methyltransferase LJ_1606"
FT                   /id="PRO_0000161985"
FT   DOMAIN          6..64
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        414
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         289
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         318
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         339
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         387
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   459 AA;  52283 MW;  524A38C616BA1216 CRC64;
     MTKFTKNKQE KNIIITIKRL GINGEGIGYY KKKIIFIPGA LPNEVVVAKI VDRHPHYLEG
     ELVRIKEKSP DRVTFPKGVD PAVGGLELAH LSYPKQLEFK QHLILESLRK YHPRDYIKYK
     VKKTIPTPNA WNYRNKAQYQ IEFNKGKSKL GLYAPNSRRL IDLPDMPTQT KETQKVEREI
     KKLIDKLHIR IADFRRHTDG IKTIAVRQSN ATGEIQVTLI TIGKKIKDLK LLASHIMKLP
     NVVSVFQNET QWQNPQVWGN KTIKLFGKSH ITEEILGKKF KLSPRAFFQL NPEQTTTLYS
     EALKYLDLTP DQTLIDAYAG VGTLGILASD QVRQVIGIES IPEAVIDAQE NCRLNHVRNA
     EYIQGNVEKL LPELKNQGVP INALIVDPPR TGLSKKLIKT LLEVKPETFV YVSCNPATLA
     KDLVLLSDAY DVRVIQPVDM MPQTPRWEGV TKLVLRKNK