ID   CAPSD_PASV1             Reviewed;         776 AA.
AC   Q9JG99; Q9JH64;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   02-JUN-2021, entry version 48.
DE   RecName: Full=Capsid polyprotein VP90;
GN   ORFNames=ORF2;
OS   Porcine astrovirus 1 (PAstV-1).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Stellavirales; Astroviridae; Mamastrovirus.
OX   NCBI_TaxID=1239567;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11424111; DOI=10.1002/jmv.1043;
RA   Wang Q.H., Kakizawa J., Wen L.Y., Shimizu M., Nishio O., Fang Z.Y.,
RA   Ushijima H.;
RT   "Genetic analysis of the capsid region of astroviruses.";
RL   J. Med. Virol. 64:245-255(2001).
CC   -!- FUNCTION: The capsid polyprotein VP90 self-assembles and undergoes a
CC       proteolytic cleavage by host caspases to yield the VP70 virions. This
CC       immature virion is composed of 180 VP70 subunits with 90 dimeric spikes
CC       and displays a T=3 icosahedral symmetry. The mature virion is obtained
CC       by further cleavages resulting in three structural proteins VP25, VP27
CC       and VP34. This forms contains only 30 spikes located on the icosahedral
CC       2-fold axes. Plays a role in the attachment to target host cell. This
CC       attachment induces virion internalization through clathrin-dependent
CC       endocytosis (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo yield mature protein(s). VP90
CC       undergoes a cascade of proteolytic cleavages presumably mediated by
CC       host caspases and extracellular proteases (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the astroviridae capsid polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; AB037272; BAA90309.1; -; Genomic_RNA.
DR   EMBL; Y15938; CAB95000.1; -; Genomic_RNA.
DR   SMR; Q9JG99; -.
DR   GO; GO:0039617; C:T=3 icosahedral viral capsid; ISS:UniProtKB.
DR   GO; GO:0075512; P:clathrin-dependent endocytosis of virus by host cell; ISS:UniProtKB.
DR   Gene3D; 2.60.120.20; -; 1.
DR   InterPro; IPR022027; Astro_capsid_p.
DR   InterPro; IPR004337; Capsid_astroviral.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF03115; Astro_capsid_N; 1.
DR   Pfam; PF12226; Astro_capsid_p; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Clathrin-mediated endocytosis of virus by host;
KW   T=3 icosahedral capsid protein; Viral penetration into host cytoplasm;
KW   Virion; Virus endocytosis by host; Virus entry into host cell.
FT   CHAIN           1..776
FT                   /note="Capsid polyprotein VP90"
FT                   /id="PRO_0000320241"
FT   REGION          1..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          648..698
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        662..680
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        681..698
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        448
FT                   /note="N -> NEEVDYDN (in Ref. 1; CAB95000)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   776 AA;  84231 MW;  24E6F14A3B9F2D84 CRC64;
     MASKSGKDVT VKVENTNGRG RSRSRSRSRS RARNKNVKIT INSKPGASGG QRRRGKPQSD
     KRVRSIVKQQ LDKSGVTGPK PAIRQRATAT LGTIGSNSSG KTELEACILT NPILVKDNTG
     NNTFGPIVAL GAQYSLWRIR FLRIKFTPMV GQSAVTGTVV RASLNPTATP SSTGWSGLGA
     RRHIDIVVGK AATFNLKASD LSGPREGWWL TNTNDSGDST LGPSIEIHTL GTTMSAYQNG
     PFTGGLFLCE LQAEWEFSGY AANPALLSLE KNRDDDAEVS FDGQQGEPLT MVVAEDSLFN
     KVATRRSTFT RGIARDGQTK SETIWQVVDT AVSAAETVVP PPFGWLIRGG YWFVKKLAGR
     TKLRNGKQTS SYVCYASYQD ALSDKPAICT GVAANFYAGR TETARANLHF TQMNEPSTGV
     GETPTAFRMY RAAPDDIVYL RFKPETVNIS VSPAARLFLA RKYTAHSLKV KGNSGTTRIH
     CVVKVNDPMW YSPDWEQVAQ PGPIPGVSLL GGTTTIGIVT AAYQAHMWGL HIATAFIVQV
     TKDINPACST SCVLTKELND QHLKTVAGQT SFNWSLRAGE TYLMMSFGAH TSAVGEWVWN
     DADISVGYTI YNSPLTPCLL LTEGSFHIVL PAKRGLIPLA STELFTVRDQ DSIPQTTEPP
     AEEDVGDNET EDESDDEDLD HFDLHDSSGS EPEDEDVENN RVTLLNTLVN QGVDLARAAK
     ISKRAYPTLA EKTRRSVFMD SLIAGCGPSS AWSEACKAAR KVSFKEPISE SRGHAE