CAPSD_PAVC
ID CAPSD_PAVC Reviewed; 584 AA.
AC P61826;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Capsid protein VP2;
DE AltName: Full=Coat protein VP2;
OS Canine parvovirus type 2 (CPV-2).
OC Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes;
OC Piccovirales; Parvoviridae; Parvovirinae; Protoparvovirus.
OX NCBI_TaxID=10788;
OH NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate CPV2b-India;
RA Gupta P.K., Rai A., Rai N., Raut A.A., Chauhan S.;
RT "Cloning of VP2 gene of canine parvovirus in a mammalian expression vector
RT for use as DNA vaccine.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Capsid protein self-assembles to form an icosahedral capsid
CC with a T=1 symmetry, about 22 nm in diameter, and consisting of 60
CC copies of two size variants of the capsid proteins, VP1 and VP2, which
CC differ by the presence of an N-terminal extension in the minor protein
CC VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are
CC responsible for the attachment to host cell receptor TFRC. This
CC attachment induces virion internalization predominantly through
CC clathrin-endocytosis. Binding to the host receptors also induces capsid
CC rearrangements leading to surface exposure of VP1 N-terminus,
CC specifically its phospholipase A2-like region and nuclear localization
CC signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach
CC the endosomal membrane during entry into host cell. Intracytoplasmic
CC transport involves microtubules and interaction between capsid proteins
CC and host dynein. Exposure of nuclear localization signal probably
CC allows nuclear import of capsids (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with host TFRC. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=VP2;
CC IsoId=P61826-1; Sequence=Displayed;
CC Name=VP1;
CC IsoId=P61826-2; Sequence=Not described;
CC -!- DOMAIN: The N-terminus of VP1 is sequestered within the mature capsid.
CC It contains a phospholipase A2-like region and nuclear localization
CC signals that might be exposed by capsid modifications during virus
CC entry (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: The capsids of autonomous parvoviruses expose a
CC proportion of VP2 N-terminus and part of that sequence can be cleaved
CC of to form VP3. {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform VP2]: Major splicing isoform produced by
CC deletion of the initiating AUG for VP1 and downstream translation of
CC VP2.
CC -!- MISCELLANEOUS: [Isoform VP1]: Minor splicing isoform. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the parvoviridae capsid protein family.
CC {ECO:0000305}.
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DR EMBL; AJ698134; CAG27358.1; -; Genomic_DNA.
DR SMR; P61826; -.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0039615; C:T=1 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0075512; P:clathrin-dependent endocytosis of virus by host cell; IEA:UniProtKB-KW.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0075521; P:microtubule-dependent intracellular transport of viral material towards nucleus; IEA:UniProtKB-KW.
DR GO; GO:0039665; P:permeabilization of host organelle membrane involved in viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0099008; P:viral entry via permeabilization of inner membrane; IEA:UniProtKB-KW.
DR GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-KW.
DR Gene3D; 2.170.30.10; -; 1.
DR InterPro; IPR016184; Capsid/spike_ssDNA_virus.
DR InterPro; IPR001403; Parvovirus_coat.
DR InterPro; IPR036952; VP1/VP2.
DR Pfam; PF00740; Parvo_coat; 1.
DR SUPFAM; SSF88645; SSF88645; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Capsid protein;
KW Clathrin-mediated endocytosis of virus by host;
KW Cytoplasmic inwards viral transport; Disulfide bond; Host nucleus;
KW Host-virus interaction; Magnesium; Metal-binding;
KW Microtubular inwards viral transport; T=1 icosahedral capsid protein;
KW Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW Viral attachment to host entry receptor;
KW Viral penetration into host cytoplasm; Viral penetration into host nucleus;
KW Viral penetration via permeabilization of host membrane; Virion;
KW Virus endocytosis by host; Virus entry into host cell.
FT CHAIN 1..584
FT /note="Capsid protein VP2"
FT /id="PRO_0000222457"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 180
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT DISULFID 490..494
FT /evidence="ECO:0000250"
SQ SEQUENCE 584 AA; 64705 MW; C2B77AB02436FBDA CRC64;
MSDGAVQPDG GQPAVRNERA TGSGNGSGGG GGGGSGGVGI STGTFNNQTE FKFLENGWVE
ITANSSRLVH LNMPESENYR RVVVNNMDKT AVNGNMALDD IHVQIVTPWS LVDANAWGVW
FNPGDWQLIV NTMSELHLVS FEQEIFNVVL KTVSESATQP PTKVYNNDLT ASLMVALDSN
NTMPFTPAAM RSETLGFYPW KPTIPTPWRY YFQWDRTLVP SHTGTSGTPT NIYHGTDPDD
VQFYTIENSV PVHLLRTGDE FATGTFFFDC KPCRLTHTWQ TNRALGLPPF LNSLPQSEGA
TNFGDIGVQQ DKRRGVTQMG NTNYITEATI MRPAEVGYSA PYYSFEASTQ GPFKTPIAAG
RGGAQTYENQ AADGDPRYAF GRQHGQKTTT TGETPDRITY IAHHDTGRYP EGDWIQNINF
NLPVTNDNVL LPTDPIGGKT GINYTNIFNT YGPLTALNNV PPVYPNGQIW DKEFDTDLKP
RPHVNAPFVC QHNCPGQLFV KVAPNLTNEY DPDASANMSR IVTYSHFWWK GKLVFKAKLR
ASHTWNPIQQ MSINVDNQFN YVPSNIGGMK IVYEKSQLAP RKLY
