ID   Y1629_SYNY3             Reviewed;         327 AA.
AC   P74346;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase slr1629;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=slr1629;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; BA000022; BAA18440.1; -; Genomic_DNA.
DR   PIR; S76181; S76181.
DR   AlphaFoldDB; P74346; -.
DR   SMR; P74346; -.
DR   IntAct; P74346; 5.
DR   STRING; 1148.1653527; -.
DR   PaxDb; P74346; -.
DR   EnsemblBacteria; BAA18440; BAA18440; BAA18440.
DR   KEGG; syn:slr1629; -.
DR   eggNOG; COG0564; Bacteria.
DR   InParanoid; P74346; -.
DR   OMA; AHPSPGW; -.
DR   PhylomeDB; P74346; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..327
FT                   /note="Uncharacterized RNA pseudouridine synthase slr1629"
FT                   /id="PRO_0000162747"
FT   DOMAIN          32..105
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        156
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   327 AA;  36037 MW;  0F3C34B1AD3AC867 CRC64;
     MIDQIIDEVT DSDPYQELLV TADGVAEGTL GVRLDKWLAE QLPELSRSRC QKLIESGQVQ
     RNGLVCQDKN LILKTGDRLV VNIPELIPLD VVAQNIPLDI LYEDEQLIII NKPAGLVVHP
     GPGHPDGTVV NALLAHCPDL AGIGGVQRPG IVHRLDKDTT GAMVVAKTEL ALHNLQVQLK
     EKTARRLYWG IVYGSPKEIQ GTVNLPVGRH PGDRQKMGIV PVEKGGREAV THWRLLERIG
     NHSWLEFQLE TGRTHQIRVH SKQMGHPLVG DNLYTSPGSV NVNLPGQALH AHQLSLIHPV
     SGEIITAIAP MPAHFEKLLV YLRQRIP