ID   Y1632_AQUAE             Reviewed;         374 AA.
AC   O67554;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Putative L-lysine 2,3-aminomutase aq_1632;
DE            Short=LAM;
DE            EC=5.4.3.-;
GN   OrderedLocusNames=aq_1632;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. KamA family.
CC       {ECO:0000305}.
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DR   EMBL; AE000657; AAC07521.1; -; Genomic_DNA.
DR   PIR; H70440; H70440.
DR   RefSeq; NP_214119.1; NC_000918.1.
DR   RefSeq; WP_010881057.1; NC_000918.1.
DR   AlphaFoldDB; O67554; -.
DR   SMR; O67554; -.
DR   STRING; 224324.aq_1632; -.
DR   PRIDE; O67554; -.
DR   EnsemblBacteria; AAC07521; AAC07521; aq_1632.
DR   KEGG; aae:aq_1632; -.
DR   PATRIC; fig|224324.8.peg.1259; -.
DR   eggNOG; COG1509; Bacteria.
DR   HOGENOM; CLU_032161_3_1_0; -.
DR   InParanoid; O67554; -.
DR   OMA; WAQFVGD; -.
DR   OrthoDB; 557227at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR003739; Lys_aminomutase/Glu_NH3_mut.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR30538; PTHR30538; 1.
DR   PIRSF; PIRSF004911; DUF160; 1.
DR   SFLD; SFLDG01070; PLP-dependent; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   TIGRFAMs; TIGR00238; TIGR00238; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Isomerase; Metal-binding; Pyridoxal phosphate;
KW   Reference proteome; S-adenosyl-L-methionine.
FT   CHAIN           1..374
FT                   /note="Putative L-lysine 2,3-aminomutase aq_1632"
FT                   /id="PRO_0000172291"
FT   DOMAIN          86..314
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         100
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         104
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         107
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         317
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   374 AA;  43371 MW;  5ADF6FFA1B9A88CB CRC64;
     MGKKLKYIID LKFIEEIPEE ERRELEKVTE KFAFRTNTYY NSLINWDNPN DPIRRIVIPT
     TEELEVWGKL DASNESKYMK VHGLEHKYPD TALLLVTDVC GIYCRFCFRK RLFMNDNDEV
     ARDVSEGLEY IRNHPEINNV LLTGGDPLIL ATFKLEKILK ALAEIPHVRI VRIGSKMLAV
     NPFRVLDDPK LLELFEWFNT ETGKKLYLMN HFNHPRELTK EARKAVELVQ KTGTTLTNQT
     PILKGINDDF ETLKTLLEEL SFIGVPPYYV FQCRPTAGNK AYSTPIEETI DLVEAVRAEV
     SGLAARVRYV MSHETGKIEI LGKTDEHIFF RYHRAADPEN RGKFMVFKRN PEAHWFDDYT
     ELVAEYKSSL SGVS