Y1677_ARATH
ID Y1677_ARATH Reviewed; 929 AA.
AC C0LGI2; Q9FXD7;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Probable LRR receptor-like serine/threonine-protein kinase At1g67720;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At1g67720; ORFNames=F12A21.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=20064227; DOI=10.1186/1471-2164-11-19;
RA Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.;
RT "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-
RT like protein kinase genes in Arabidopsis thaliana.";
RL BMC Genomics 11:19-19(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- INTERACTION:
CC C0LGI2; Q9LIG2: At3g21340; NbExp=2; IntAct=EBI-17070892, EBI-941096;
CC C0LGI2; Q9LRT1: At3g28040; NbExp=2; IntAct=EBI-17070892, EBI-16956175;
CC C0LGI2; Q8GX94: LRR-RLK; NbExp=2; IntAct=EBI-17070892, EBI-16955556;
CC C0LGI2; Q9LZV7: PXC2; NbExp=2; IntAct=EBI-17070892, EBI-1238200;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG28906.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC008113; AAG28906.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34687.1; -; Genomic_DNA.
DR EMBL; FJ708672; ACN59267.1; -; mRNA.
DR RefSeq; NP_564904.1; NM_105440.2.
DR AlphaFoldDB; C0LGI2; -.
DR SMR; C0LGI2; -.
DR BioGRID; 28318; 30.
DR IntAct; C0LGI2; 30.
DR STRING; 3702.AT1G67720.1; -.
DR PaxDb; C0LGI2; -.
DR PRIDE; C0LGI2; -.
DR ProteomicsDB; 243033; -.
DR EnsemblPlants; AT1G67720.1; AT1G67720.1; AT1G67720.
DR GeneID; 843097; -.
DR Gramene; AT1G67720.1; AT1G67720.1; AT1G67720.
DR KEGG; ath:AT1G67720; -.
DR Araport; AT1G67720; -.
DR TAIR; locus:2008565; AT1G67720.
DR eggNOG; ENOG502QSBF; Eukaryota.
DR HOGENOM; CLU_000288_41_3_1; -.
DR InParanoid; C0LGI2; -.
DR OMA; CVEQHGV; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; C0LGI2; -.
DR PRO; PR:C0LGI2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; C0LGI2; baseline and differential.
DR Genevisible; C0LGI2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR024788; Malectin-like_Carb-bd_dom.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF12819; Malectin_like; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Glycoprotein; Kinase; Leucine-rich repeat; Membrane;
KW Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat;
KW Serine/threonine-protein kinase; Signal; Transferase; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..929
FT /note="Probable LRR receptor-like serine/threonine-protein
FT kinase At1g67720"
FT /id="PRO_0000387539"
FT TOPO_DOM 22..531
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 532..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 553..929
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 413..437
FT /note="LRR 1"
FT REPEAT 438..460
FT /note="LRR 2"
FT REPEAT 461..484
FT /note="LRR 3"
FT REPEAT 485..508
FT /note="LRR 4"
FT DOMAIN 606..880
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 731
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 612..620
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 634
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 679
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 735
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 764
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 770
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 778
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 236
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 293
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 320
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 407
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 494
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 929 AA; 103753 MW; AC460DA336D01A4E CRC64;
MGLCLAQLAV TCLFLVPFVL SQVTEFVSID CGCSSNYTDP RTGLGWVSDS EIIKQGKPVT
LANTNWNSMQ YRRRRDFPTD NKKYCYRLST KERRRYIVRT TFLYGGLGSE EAYPKFQLYL
DATKWATVTI QEVSRVYVEE LIVRATSSYV DVCVCCAITG SPFMSTLELR PLNLSMYATD
YEDNFFLKVA ARVNFGAPNM DALRYPDDPY DRIWESDINK RPNYLVGVAP GTTRINTSKT
INTLTREYPP MKVMQTAVVG TQGLISYRLN LEDFPANARA YAYFAEIEEL GANETRKFKL
VQPYFPDYSN AVVNIAENAN GSYTLYEPSY MNVTLDFVLT FSFGKTKDST QGPLLNAIEI
SKYLPISVKT DRSDVSVLDA IRSMSPDSDW ASEGGDPCIP VLWSWVNCSS TSPPRVTKIA
LSRKNLRGEI PPGINYMEAL TELWLDDNEL TGTLPDMSKL VNLKIMHLEN NQLSGSLPPY
LAHLPNLQEL SIENNSFKGK IPSALLKGKV LFKYNNNPEL QNEAQRKHFW QILGISIAAV
AILLLLVGGS LVLLCALRKT KRADKGDSTE TKKKGLVAYS AVRGGHLLDE GVAYFISLPV
LEEATDNFSK KVGRGSFGSV YYGRMKDGKE VAVKITADPS SHLNRQFVTE VALLSRIHHR
NLVPLIGYCE EADRRILVYE YMHNGSLGDH LHGSSDYKPL DWLTRLQIAQ DAAKGLEYLH
TGCNPSIIHR DVKSSNILLD INMRAKVSDF GLSRQTEEDL THVSSVAKGT VGYLDPEYYA
SQQLTEKSDV YSFGVVLFEL LSGKKPVSAE DFGPELNIVH WARSLIRKGD VCGIIDPCIA
SNVKIESVWR VAEVANQCVE QRGHNRPRMQ EVIVAIQDAI RIERGNENGL KSSSSSSSKA
QSSRKTLLTS FLELESPDIS RNSLAPAAR
