ID   Y1682_STRP6             Reviewed;         419 AA.
AC   Q5X9U6;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Putative zinc metalloprotease M6_Spy1682;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=M6_Spy1682;
OS   Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=286636;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-946 / MGAS10394;
RX   PubMed=15272401; DOI=10.1086/422697;
RA   Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E.,
RA   Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
RT   "Progress toward characterization of the group A Streptococcus metagenome:
RT   complete genome sequence of a macrolide-resistant serotype M6 strain.";
RL   J. Infect. Dis. 190:727-738(2004).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; CP000003; AAT87817.1; -; Genomic_DNA.
DR   RefSeq; WP_011184987.1; NC_006086.1.
DR   AlphaFoldDB; Q5X9U6; -.
DR   SMR; Q5X9U6; -.
DR   EnsemblBacteria; AAT87817; AAT87817; M6_Spy1682.
DR   KEGG; spa:M6_Spy1682; -.
DR   HOGENOM; CLU_025778_1_0_9; -.
DR   OMA; QYMVGFG; -.
DR   Proteomes; UP000001167; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF13180; PDZ_2; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Metal-binding; Metalloprotease;
KW   Protease; Transmembrane; Transmembrane helix; Zinc.
FT   CHAIN           1..419
FT                   /note="Putative zinc metalloprotease M6_Spy1682"
FT                   /id="PRO_0000088470"
FT   TRANSMEM        169..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        343..365
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          175..274
FT                   /note="PDZ"
FT   ACT_SITE        19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         18
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         22
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   419 AA;  45620 MW;  3944087416275A75 CRC64;
     MLGIITFIII FGILVIVHEF GHFYFAKKSG ILVREFAIGM GPKIFSHVDQ GGTLYTLRML
     PLGGYVRMAG WGDDKTEIKT GTPASLTLNE QGFVKRINLS QSKLDPTSLP MHVTGYDLED
     QLSITGLVLE ETKTYKVAHD ATIVEEDGTE IRIAPLDVQY QNASIGGRLI TNFAGPMNNF
     ILGIVVFILL VFLQGGMPDF SSNHVGVQEN GAAAKAGLRD NDQIVAINGY KVTSWNDLTE
     AVDLATRDLG PSQTIKVTYK SHQRLKTVAV KPQKHAKTYT IGVKASLKTG FKDKLLGGLE
     LAWSGAFTIL NALKGLITGF SLNKLGGPVA MYDMSNQAAQ NGLESVLSLM AMLSINLGIF
     NLIPIPALDG GKILMNIIEA IRRKPIKQET EAYITLAGVA IMVVLMIAVT WNDIMRVFF