Y1683_ARCFU
ID Y1683_ARCFU Reviewed; 156 AA.
AC O28590;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=VapC ribonuclease AF_1683;
DE Short=RNase AF_1683;
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Putative toxin AF_1683;
GN OrderedLocusNames=AF_1683;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
RG Midwest center for structural genomics (MCSG);
RT "The structure of gene product AF1683 from Archaeoglobus fulgidus.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC RNase. {ECO:0000255|HAMAP-Rule:MF_00265}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; AE000782; AAB89566.1; -; Genomic_DNA.
DR PIR; B69460; B69460.
DR RefSeq; WP_010879179.1; NC_000917.1.
DR PDB; 1W8I; X-ray; 2.10 A; A=1-156.
DR PDBsum; 1W8I; -.
DR AlphaFoldDB; O28590; -.
DR SMR; O28590; -.
DR STRING; 224325.AF_1683; -.
DR EnsemblBacteria; AAB89566; AAB89566; AF_1683.
DR GeneID; 24795426; -.
DR KEGG; afu:AF_1683; -.
DR eggNOG; arCOG04502; Archaea.
DR HOGENOM; CLU_1682583_0_0_2; -.
DR OrthoDB; 96415at2157; -.
DR EvolutionaryTrace; O28590; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF01850; PIN; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..156
FT /note="VapC ribonuclease AF_1683"
FT /id="PRO_0000128047"
FT DOMAIN 4..125
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 6
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 103
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 7..14
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 21..32
FT /evidence="ECO:0007829|PDB:1W8I"
FT TURN 33..36
FT /evidence="ECO:0007829|PDB:1W8I"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 43..55
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 60..67
FT /evidence="ECO:0007829|PDB:1W8I"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:1W8I"
FT STRAND 71..76
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 80..92
FT /evidence="ECO:0007829|PDB:1W8I"
FT TURN 93..95
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 101..113
FT /evidence="ECO:0007829|PDB:1W8I"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:1W8I"
FT TURN 131..136
FT /evidence="ECO:0007829|PDB:1W8I"
FT HELIX 139..151
FT /evidence="ECO:0007829|PDB:1W8I"
SQ SEQUENCE 156 AA; 17841 MW; F6B4286D1C96A6B4 CRC64;
MAALIDTGIF FGFYSLKDVH HMDSVAIVVH AVEGKWGRLF VTNHILDETL TLLKYKKLPA
DKFLEGFVES GVLNIIYTDD EVERKALEVF KARVYEKGFS YTDAISEVVA EELKLKLISY
DSRFSLPTIG RDYWKSLDES ERKRISAILR EKGIDG
