ID   Y1698_LACJO             Reviewed;         450 AA.
AC   Q74I68;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Uncharacterized RNA methyltransferase LJ_1698;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=LJ_1698;
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533;
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017198; AAS09471.1; -; Genomic_DNA.
DR   RefSeq; WP_011162379.1; NC_005362.1.
DR   AlphaFoldDB; Q74I68; -.
DR   SMR; Q74I68; -.
DR   STRING; 257314.LJ_1698; -.
DR   PRIDE; Q74I68; -.
DR   EnsemblBacteria; AAS09471; AAS09471; LJ_1698.
DR   KEGG; ljo:LJ_1698; -.
DR   PATRIC; fig|257314.6.peg.1523; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; SIIHVMN; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..450
FT                   /note="Uncharacterized RNA methyltransferase LJ_1698"
FT                   /id="PRO_0000161986"
FT   DOMAIN          1..58
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        405
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         280
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         309
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         330
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         378
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   450 AA;  50712 MW;  E473ADC51DE7B7C8 CRC64;
     MQKNQIVDLE ITDLSYEAMG VAHLDGMTVF VNNALPGEIV SAKLLKVKKN FAFAKIEKIK
     KESPDRINVK LRQWVQTGLA SLAHIKYDKQ LEFKRNQVVN LLHKADLDNV KVGQTMPSPE
     ETGYRNKAQV PVREVNGKLD IGFFRKHSHD LVPLTSFFTT DPEIDRVLIK VRDILRKNHV
     PAYDEIHNKG EVRYLEVRRS KATGEIMVIL VCLHKDFPQL KEVTKEISEI KGVTSVVLNH
     NPKKTNVILG KKDYLLWGEP QITDKIGDVS FKISPQSFFQ INSLQTPRLY DLAIQKADLK
     PDDVVIDAYS GIGTIGLSVA KHVKAVRGME VIEPAVEDAN ANAKLNGITN AEYVVGKAEE
     VMPRWAKEGL KTDVIFVDPP RKGLTPEFID AAVETNPKKI VYISCNPATM VRDLQLFKEQ
     GYDFDEIDPV DMFPQTPHVE AVAVLERTEK