Y1738_MYCBO
ID Y1738_MYCBO Reviewed; 254 AA.
AC P65843; A0A1R3XZ33; O33210; X2BI85;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase Mb1738;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=BQ2027_MB1738;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIU00342.1; -; Genomic_DNA.
DR RefSeq; NP_855391.1; NC_002945.3.
DR RefSeq; WP_003408439.1; NC_002945.4.
DR AlphaFoldDB; P65843; -.
DR SMR; P65843; -.
DR EnsemblBacteria; SIU00342; SIU00342; BQ2027_MB1738.
DR PATRIC; fig|233413.5.peg.1896; -.
DR OMA; HVPKTYE; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; RNA-binding.
FT CHAIN 1..254
FT /note="Uncharacterized RNA pseudouridine synthase Mb1738"
FT /id="PRO_0000100030"
FT DOMAIN 14..81
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 119
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 254 AA; 27597 MW; EB2D95D7CF0FDF26 CRC64;
MMAEPEESRE PRGIRLQKVL SQAGIASRRA AEKMIVDGRV EVDGHVVTEL GTRVDPQVAV
VRVDGARVVL DDSLVYLALN KPRGMHSTMS DDRGRPCIGD LIERKVRGTK KLFHVGRLDA
DTEGLMLLTN DGELAHRLMH PSHEVPKTYL ATVTGSVPRG LGRTLRAGIE LDDGPAFVDD
FAVVDAIPGK TLVRVTLHEG RNRIVRRLLA AAGFPVEALV RTDIGAVSLG KQRPGSVRAL
RSNEIGQLYQ AVGL