Y1755_ARATH
ID Y1755_ARATH Reviewed; 864 AA.
AC C0LGD8; Q9LNX8;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Probable LRR receptor-like serine/threonine-protein kinase At1g07550;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At1g07550; ORFNames=F22G5.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=20064227; DOI=10.1186/1471-2164-11-19;
RA Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.;
RT "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-
RT like protein kinase genes in Arabidopsis thaliana.";
RL BMC Genomics 11:19-19(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- INTERACTION:
CC C0LGD8; Q9LZV7: PXC2; NbExp=2; IntAct=EBI-16907406, EBI-1238200;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79546.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC022464; AAF79546.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28141.1; -; Genomic_DNA.
DR EMBL; FJ708627; ACN59223.1; -; mRNA.
DR RefSeq; NP_172235.2; NM_100629.4.
DR AlphaFoldDB; C0LGD8; -.
DR SMR; C0LGD8; -.
DR BioGRID; 22510; 41.
DR IntAct; C0LGD8; 41.
DR STRING; 3702.AT1G07550.1; -.
DR iPTMnet; C0LGD8; -.
DR MetOSite; C0LGD8; -.
DR PaxDb; C0LGD8; -.
DR PRIDE; C0LGD8; -.
DR ProteomicsDB; 243055; -.
DR EnsemblPlants; AT1G07550.1; AT1G07550.1; AT1G07550.
DR GeneID; 837269; -.
DR Gramene; AT1G07550.1; AT1G07550.1; AT1G07550.
DR KEGG; ath:AT1G07550; -.
DR Araport; AT1G07550; -.
DR TAIR; locus:2024987; AT1G07550.
DR eggNOG; ENOG502QQCZ; Eukaryota.
DR HOGENOM; CLU_000288_41_1_1; -.
DR InParanoid; C0LGD8; -.
DR OMA; TSYWHEV; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; C0LGD8; -.
DR PRO; PR:C0LGD8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; C0LGD8; baseline and differential.
DR Genevisible; C0LGD8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR024788; Malectin-like_Carb-bd_dom.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF12819; Malectin_like; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Glycoprotein; Kinase; Leucine-rich repeat; Membrane;
KW Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat;
KW Serine/threonine-protein kinase; Signal; Transferase; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..864
FT /note="Probable LRR receptor-like serine/threonine-protein
FT kinase At1g07550"
FT /id="PRO_0000387543"
FT TOPO_DOM 24..507
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 529..864
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 411..434
FT /note="LRR 1"
FT REPEAT 435..457
FT /note="LRR 2"
FT REPEAT 459..480
FT /note="LRR 3"
FT DOMAIN 560..831
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 684
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 566..574
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 587
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 551
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 632
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 718
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 723
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 731
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 289
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 432
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 445
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 464
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 864 AA; 96785 MW; E9498ECFC225F70F CRC64;
MDTCTRLLFA ACATLSILHL VQSQNQQGFI SLDCGLASNE SPYNEANSNL TYISDADFIQ
GGKTGNVQKD LLMKLRKPYT VLRYFPDGIR NCYSLNVKQD TNYLIRVMFR YGNYDGLNNS
PRFDLYLGPN IWTTIDMGKS GDGVLEEIIH ITRSNILDIC LVKTGTSTPM ISSIELRPLL
YDTYIAQTGS LRNYNRFYFT DSNNYIRYPQ DVHDRIWVPL ILPEWTHINT SHHVIDSIDG
YDPPQDVLRT GAMPANASDP MTITWNLKTA TDQVYGYIYI AEIMEVQANE TREFEVVVNN
KVHFDPFRPT RFEAQVMFNN VPLTCEGGFC RLQLIKTPKS TLPPLMNAFE IFTGIEFPQS
ETNQNDVIAV KNIQASYGLN RISWQGDPCV PKQFLWTGLS CNVIDVSTPP RIVKLDLSSS
GLNGVIPPSI QNLTQLQELD LSQNNLTGKV PEFLAKMKYL LVINLSGNKL SGLVPQALLD
RKKEGLKLLV DENMICVSCG TRFPTAAVAA SVSAVAIIIL VLVLIFVLRR RKPSAGKVTR
SSFKSENRRF TYSDVNKMTN NFQVVIGKGG FGVVYQGCLN NEQAAIKVLS HSSAQGYKEF
KTEVELLLRV HHEKLVSLIG YCDDDNGLAL IYELMGKGNL KEHLSGKPGC SVLSWPIRLK
IALESAIGIE YLHTGCKPKI VHRDVKSTNI LLSEEFEAKI ADFGLSRSFL IGNEAQPTVV
AGTFGYLDPE YHKTSLLSMK SDVYSFGVVL LEIISGQDVI DLSRENCNIV EWTSFILENG
DIESIVDPNL HQDYDTSSAW KVVELAMSCV NRTSKERPNM SQVVHVLNEC LETCEKWRKS
QEVDLSSPLE LSIVVDTEIN PKAR
