ID   Y1758_AQUAE             Reviewed;         316 AA.
AC   O67638;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase aq_1758;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=aq_1758;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE000657; AAC07603.1; -; Genomic_DNA.
DR   PIR; D70451; D70451.
DR   RefSeq; NP_214204.1; NC_000918.1.
DR   RefSeq; WP_010881141.1; NC_000918.1.
DR   AlphaFoldDB; O67638; -.
DR   SMR; O67638; -.
DR   STRING; 224324.aq_1758; -.
DR   EnsemblBacteria; AAC07603; AAC07603; aq_1758.
DR   KEGG; aae:aq_1758; -.
DR   PATRIC; fig|224324.8.peg.1356; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_4_0; -.
DR   InParanoid; O67638; -.
DR   OMA; AHPSPGW; -.
DR   OrthoDB; 1714699at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..316
FT                   /note="Uncharacterized RNA pseudouridine synthase aq_1758"
FT                   /id="PRO_0000162727"
FT   DOMAIN          16..89
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        140
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   316 AA;  36537 MW;  74ACC60DAACACF31 CRC64;
     MKISEVLEFK VEGEEERLDK FLARAYPDFS RSYIKKLVKE GLVYVNGEEV RKPSRKLREG
     ERVILHVPEP EPLDVKPENI PINIIYEDED IAVVEKPCGL VVHPSPGYTS GTLVNALLYH
     IKDLSSIGGV ERPGIVHRLD KETAGVMVIA KNNTAHRNLV RQFQERKTEK FYKVLVKGLV
     KKDYGAIDTP IARHPVDRKR FWVRKEGKEA LTEYWVLKRY EKYEITLLKV KIHTGRTHQI
     RVHFASIGHP VLGDRTYGFK SSSVPKELLS LMGECNMLIA YHLGFYHPTK GEWMVFEIEE
     PETFKSVYSW LEEHSP