CAPSD_TMV
ID CAPSD_TMV Reviewed; 159 AA.
AC P69687; P03570;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Capsid protein;
DE AltName: Full=Coat protein;
GN Name=CP;
OS Tobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain
OS U1)).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Tobamovirus.
OX NCBI_TaxID=12243;
OH NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=6964389; DOI=10.1073/pnas.79.19.5818;
RA Goelet P., Lomonossoff G.P., Butler P.J.G., Akam M.E., Gait M.J., Karn J.;
RT "Nucleotide sequence of tobacco mosaic virus RNA.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:5818-5822(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=109810; DOI=10.1093/nar/6.4.1287;
RA Guilley H., Jonard G., Kukla B., Richards K.E.;
RT "Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.";
RL Nucleic Acids Res. 6:1287-1308(1979).
RN [3]
RP PRELIMINARY PROTEIN SEQUENCE OF 2-159, AND ACETYLATION AT SER-2.
RX PubMed=16590772; DOI=10.1073/pnas.46.11.1463;
RA Tsugita A., Gish D.T., Young J., Fraenkel-Conrat H., Knight C.A.,
RA Stanley W.M.;
RT "The complete amino acid sequence of the protein of tobacco mosaic virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 46:1463-1469(1960).
RN [4]
RP PROTEIN SEQUENCE OF 2-42 AND 94-113, AND SEQUENCE REVISION.
RX PubMed=14165501; DOI=10.1016/0003-9861(64)90232-2;
RA Funatsu G., Tsugita A., Fraenkel-Conrat H.;
RT "Studies on the amino acid sequence of tobacco mosaic virus protein. V.
RT Amino acid sequences of two peptides from tryptic digests and location of
RT amide group.";
RL Arch. Biochem. Biophys. 105:25-41(1964).
RN [5]
RP PROTEIN SEQUENCE OF 2-159.
RA Anderer F.A., Handschuh D.;
RT "The sequence of amino acids in the protein of tobacco mosaic virus. IV.
RT Separation of tobacco mosaic virus protein with trypsin.";
RL Z. Naturforsch. B 17:536-543(1962).
RN [6]
RP SEQUENCE REVISION.
RX PubMed=4381389;
RA Anderer F.A., Wittmann-Liebold B., Wittmann H.G.;
RT "Further studies on amino acid sequence of proteins in tobacco mosaic
RT virus.";
RL Z. Naturforsch. B 20:1203-1213(1965).
RN [7]
RP SEQUENCE REVISION.
RX PubMed=5452764;
RA Nozu Y., Ohno T., Okada Y.;
RT "Amino acid sequences of some common Japanese strains of tobacco mosaic
RT virus.";
RL J. Biochem. 68:39-52(1970).
RN [8]
RP MUTANTS DEFECTIVE CAPSID PM2; DT1 AND DT2.
RX PubMed=8341610; DOI=10.1093/nar/21.14.3325;
RA Kuhlmann U., Sarkar S., Rohde W.;
RT "Capsid protein gene sequences of four tobacco mosaic virus strains
RT defective for virus assembly.";
RL Nucleic Acids Res. 21:3325-3325(1993).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) OF 1-159.
RX PubMed=3952490; DOI=10.1126/science.3952490;
RA Namba K., Stubbs G.;
RT "Structure of tobacco mosaic virus at 3.6-A resolution: implications for
RT assembly.";
RL Science 231:1401-1406(1986).
CC -!- FUNCTION: Capsid protein self-assembles to form rod-shaped virions
CC about 18 nm in diameter with a central canal enclosing the viral
CC genomic RNA.
CC -!- INTERACTION:
CC P69687; P69687: CP; NbExp=2; IntAct=EBI-15927445, EBI-15927445;
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the virgaviridae capsid protein family.
CC {ECO:0000305}.
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DR EMBL; V01408; -; NOT_ANNOTATED_CDS; Unassigned_RNA.
DR EMBL; V01409; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; V01406; CAA24685.1; -; Genomic_RNA.
DR EMBL; X70882; CAA50227.1; -; mRNA.
DR EMBL; X70883; CAA50228.1; -; mRNA.
DR EMBL; X70884; CAA50229.1; -; mRNA.
DR RefSeq; NP_597750.1; NC_001367.1.
DR PDB; 1EI7; X-ray; 2.45 A; A/B=2-159.
DR PDB; 2TMV; Fiber; 2.90 A; P=2-159.
DR PDB; 3J06; EM; -; A=1-159.
DR PDB; 3KML; X-ray; 3.01 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q=2-100.
DR PDB; 4GQH; X-ray; 3.06 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/h/i=2-155.
DR PDB; 4UDV; EM; 3.35 A; A=2-159.
DR PDB; 6I5A; EM; 2.30 A; A=1-159.
DR PDB; 6R7M; EM; 1.92 A; A=2-154.
DR PDB; 6SAE; EM; 1.90 A; A=2-159.
DR PDB; 6SAG; EM; 2.00 A; A=2-159.
DR PDB; 6X0Q; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q=2-158.
DR PDB; 6X0R; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q=2-158.
DR PDBsum; 1EI7; -.
DR PDBsum; 2TMV; -.
DR PDBsum; 3J06; -.
DR PDBsum; 3KML; -.
DR PDBsum; 4GQH; -.
DR PDBsum; 4UDV; -.
DR PDBsum; 6I5A; -.
DR PDBsum; 6R7M; -.
DR PDBsum; 6SAE; -.
DR PDBsum; 6SAG; -.
DR PDBsum; 6X0Q; -.
DR PDBsum; 6X0R; -.
DR SMR; P69687; -.
DR DIP; DIP-59677N; -.
DR iPTMnet; P69687; -.
DR ABCD; P69687; 6 sequenced antibodies.
DR GeneID; 1494080; -.
DR KEGG; vg:1494080; -.
DR EvolutionaryTrace; P69687; -.
DR Proteomes; UP000000522; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR Gene3D; 1.20.120.70; -; 1.
DR InterPro; IPR001337; TMV-like_coat.
DR InterPro; IPR036417; TMV-like_coat_sf.
DR Pfam; PF00721; TMV_coat; 1.
DR SUPFAM; SSF47195; SSF47195; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Capsid protein; Direct protein sequencing;
KW Helical capsid protein; Reference proteome; Virion.
FT INIT_MET 1
FT /note="Removed; by host"
FT /evidence="ECO:0000269|PubMed:14165501, ECO:0000269|Ref.5"
FT CHAIN 2..159
FT /note="Capsid protein"
FT /id="PRO_0000144918"
FT MOD_RES 2
FT /note="N-acetylserine; by host"
FT /evidence="ECO:0000269|PubMed:16590772"
FT VARIANT 29
FT /note="T -> I (in PM2; DT1 and DT2)"
FT VARIANT 79
FT /note="P -> S (in DT2)"
FT VARIANT 96
FT /note="E -> D (in PM2; DT1 and DT2)"
FT VARIANT 104
FT /note="T -> M (in PM2)"
FT VARIANT 138
FT /note="G -> R (in DT1 and DT2)"
FT VARIANT 149
FT /note="S -> Y (in DT2)"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:6X0Q"
FT HELIX 8..14
FT /evidence="ECO:0007829|PDB:6SAE"
FT HELIX 21..31
FT /evidence="ECO:0007829|PDB:6SAE"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:3J06"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:3J06"
FT HELIX 39..51
FT /evidence="ECO:0007829|PDB:6SAE"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:6R7M"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:6SAE"
FT HELIX 77..87
FT /evidence="ECO:0007829|PDB:6SAE"
FT HELIX 93..97
FT /evidence="ECO:0007829|PDB:6SAE"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:6R7M"
FT HELIX 105..134
FT /evidence="ECO:0007829|PDB:6SAE"
FT TURN 135..138
FT /evidence="ECO:0007829|PDB:6SAE"
FT HELIX 142..148
FT /evidence="ECO:0007829|PDB:6SAE"
FT STRAND 152..154
FT /evidence="ECO:0007829|PDB:4GQH"
SQ SEQUENCE 159 AA; 17624 MW; 5C90E8478A43DBC4 CRC64;
MSYSITTPSQ FVFLSSAWAD PIELINLCTN ALGNQFQTQQ ARTVVQRQFS EVWKPSPQVT
VRFPDSDFKV YRYNAVLDPL VTALLGAFDT RNRIIEVENQ ANPTTAETLD ATRRVDDATV
AIRSAINNLI VELIRGTGSY NRSSFESSSG LVWTSGPAT
