ID   Y1760_MYCTO             Reviewed;         502 AA.
AC   P9WKB8; L0T7L6; O06795;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT1809;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT1809;
GN   OrderedLocusNames=MT1809;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK46080.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK46080.1; ALT_INIT; Genomic_DNA.
DR   PIR; A70988; A70988.
DR   RefSeq; WP_003899007.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKB8; -.
DR   SMR; P9WKB8; -.
DR   EnsemblBacteria; AAK46080; AAK46080; MT1809.
DR   KEGG; mtc:MT1809; -.
DR   PATRIC; fig|83331.31.peg.1944; -.
DR   HOGENOM; CLU_024186_4_2_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..502
FT                   /note="Putative diacyglycerol O-acyltransferase MT1809"
FT                   /id="PRO_0000427681"
FT   ACT_SITE        174
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   502 AA;  54062 MW;  C821223B46B72CF8 CRC64;
     MPRGCAGARF ACNACLNFLA GLGISEPISP GWAAMERLSG LDAFFLYMET PSQPLNVCCV
     LELDTSTMPG GYTYGRFHAA LEKYVKAAPE FRMKLADTEL NLDHPVWVDD DNFQIRHHLR
     RVAMPAPGGR RELAEICGYI AGLPLDRDRP LWEMWVIEGG ARSDTVAVML KVHHAVVDGV
     AGANLLSHLC SLQPDAPAPQ PVRGTGGGNV LQIAASGLVG FASRPVRLAT VVPATVLTLV
     RTLLRAREGR TMAAPFSAPP TPFNGPLGRL RNIAYTQLDM RDVKRVKDRF GVTINDVVVA
     LCAGALRRFL LEHGVLPEAP LVATVPVSVH DKSDRPGRNQ ATWMFCRVPS QISDPAQRIR
     TIAAGNTVAK DHAAAIGPTL LHDWIQFGGS TMFGAAMRIL PHISITHSPA YNLILSNVPG
     PQAQLYFLGC RMDSMFPLGP LLGNAGLNIT VMSLNGELGV GIVSCPDLLP DLWGVADGFP
     EALKELLECS DDQPEGSNHQ DS