Y1828_MYCTU
ID Y1828_MYCTU Reviewed; 247 AA.
AC P9WME7; L0TAQ6; P67669; Q50605;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Uncharacterized HTH-type transcriptional regulator Rv1828 {ECO:0000305};
GN OrderedLocusNames=Rv1828; ORFNames=MTCY1A11.15c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4] {ECO:0007744|PDB:5YDC, ECO:0007744|PDB:5YDD}
RP X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 127-247, FUNCTION, DNA-BINDING,
RP SUBUNIT, INDUCTION, AND DOMAIN.
RX PubMed=30306715; DOI=10.1111/febs.14676;
RA Singh S., Sevalkar R.R., Sarkar D., Karthikeyan S.;
RT "Characteristics of the essential pathogenicity factor Rv1828, a MerR
RT family transcription regulator from Mycobacterium tuberculosis.";
RL FEBS J. 285:4424-4444(2018).
CC -!- FUNCTION: Transcriptional regulator that binds to its own promoter and
CC thus may play a role in the regulation of the cotranscribed genes
CC Rv1827 and Rv1828. Can also bind several promoter regions of genes that
CC are essential, including ftsZ. Binds to the imperfect everted repeat
CC sequence CTCAA through its winged-HTH motif.
CC {ECO:0000269|PubMed:30306715}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:30306715}.
CC -!- INDUCTION: Autoregulated. {ECO:0000269|PubMed:30306715}.
CC -!- DOMAIN: Contains an N-terminal DNA-binding domain, a linker region and
CC a C-terminal effector binding domain. Dimerization is mediated via the
CC C-terminal domain. {ECO:0000269|PubMed:30306715}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC {ECO:0000269|PubMed:19099550}.
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DR EMBL; AL123456; CCP44594.1; -; Genomic_DNA.
DR PIR; E70721; E70721.
DR RefSeq; NP_216344.1; NC_000962.3.
DR RefSeq; WP_003409239.1; NZ_NVQJ01000013.1.
DR PDB; 5YDC; X-ray; 1.91 A; A/B=127-247.
DR PDB; 5YDD; X-ray; 1.50 A; A/B=127-247.
DR PDBsum; 5YDC; -.
DR PDBsum; 5YDD; -.
DR AlphaFoldDB; P9WME7; -.
DR SMR; P9WME7; -.
DR STRING; 83332.Rv1828; -.
DR PaxDb; P9WME7; -.
DR DNASU; 885336; -.
DR GeneID; 45425802; -.
DR GeneID; 885336; -.
DR KEGG; mtu:Rv1828; -.
DR TubercuList; Rv1828; -.
DR eggNOG; COG0789; Bacteria.
DR OMA; NPQTRAH; -.
DR PhylomeDB; P9WME7; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR000551; MerR-type_HTH_dom.
DR Pfam; PF00376; MerR; 1.
DR SMART; SM00422; HTH_MERR; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
DR PROSITE; PS50937; HTH_MERR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..247
FT /note="Uncharacterized HTH-type transcriptional regulator
FT Rv1828"
FT /id="PRO_0000098168"
FT DOMAIN 11..85
FT /note="HTH merR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT DNA_BIND 14..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT HELIX 130..137
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 141..149
FT /evidence="ECO:0007829|PDB:5YDD"
FT STRAND 159..161
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 163..177
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 182..185
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 186..203
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 204..209
FT /evidence="ECO:0007829|PDB:5YDD"
FT HELIX 220..244
FT /evidence="ECO:0007829|PDB:5YDD"
SQ SEQUENCE 247 AA; 26443 MW; C3BA9AE7E08B3D37 CRC64;
MSAPDSPALA GMSIGAVLDL LRPDFPDVTI SKIRFLEAEG LVTPRRASSG YRRFTAYDCA
RLRFILTAQR DHYLPLKVIR AQLDAQPDGE LPPFGSPYVL PRLVPVAGDS AGGVGSDTAS
VSLTGIRLSR EDLLERSEVA DELLTALLKA GVITTGPGGF FDEHAVVILQ CARALAEYGV
EPRHLRAFRS AADRQSDLIA QIAGPLVKAG KAGARDRADD LAREVAALAI TLHTSLIKSA
VRDVLHR
