Y1839_PSEAE
ID Y1839_PSEAE Reviewed; 346 AA.
AC Q9I2Q6;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable RNA methyltransferase PA1839;
DE EC=2.1.1.-;
GN OrderedLocusNames=PA1839;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RlmN family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG05228.1; -; Genomic_DNA.
DR PIR; A83415; A83415.
DR RefSeq; NP_250530.1; NC_002516.2.
DR RefSeq; WP_003088017.1; NZ_QZGE01000003.1.
DR AlphaFoldDB; Q9I2Q6; -.
DR SMR; Q9I2Q6; -.
DR STRING; 287.DR97_38; -.
DR PaxDb; Q9I2Q6; -.
DR PRIDE; Q9I2Q6; -.
DR EnsemblBacteria; AAG05228; AAG05228; PA1839.
DR GeneID; 880671; -.
DR KEGG; pae:PA1839; -.
DR PATRIC; fig|208964.12.peg.1912; -.
DR PseudoCAP; PA1839; -.
DR HOGENOM; CLU_029101_3_3_6; -.
DR InParanoid; Q9I2Q6; -.
DR OMA; KVVFMGM; -.
DR PhylomeDB; Q9I2Q6; -.
DR BioCyc; PAER208964:G1FZ6-1878-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR040072; Methyltransferase_A.
DR InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN/Cfr.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR30544; PTHR30544; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF006004; CHP00048; 1.
DR SFLD; SFLDF00275; adenosine_C2_methyltransferase; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Cytoplasm; Disulfide bond; Iron; Iron-sulfur; Metal-binding;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..346
FT /note="Probable RNA methyltransferase PA1839"
FT /id="PRO_0000350330"
FT DOMAIN 94..320
FT /note="Radical SAM core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT ACT_SITE 91
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT ACT_SITE 325
FT /note="S-methylcysteine intermediate"
FT /evidence="ECO:0000250"
FT BINDING 108
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000250"
FT BINDING 115
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000250"
FT BINDING 153..154
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 183
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 206..208
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 282
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT DISULFID 101..325
FT /note="(transient)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 346 AA; 38090 MW; B180006A521947BB CRC64;
MRSQDLHQRL ADLGAKPLHC GRIVRAWLQG RALDACTARQ RAEDFLPLGV RHGLPQVAAE
LEGIARLHSE HPASDGSSRL LVELADRQMV ESVLLPRGGL CVSTQVGCAV GCVFCMTGRS
GLLRQVGSLE MVAQVVLARR RRAVKKVVFM GMGEPAHNLD NVLEAIDLLG TDGGIGHKNL
VFSTVGDPRV FERLPRQRVK PALALSLHST RAELRRQLLP KAPPLSPEEL VEAGEAYARR
VDYPIQYQWT LLEGINDSLE EMDGILRLLK GRFAVMNLIP YNSMDGDAYR RPSGERIVEL
VRYLHSRGVL TKVRNSAGQD IDGGCGQLRA RATQGTAERR IPARQA
