Y1864_ARATH
ID Y1864_ARATH Reviewed; 427 AA.
AC Q0V7T5; A8MSF7; Q38943; Q9M8N1;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Probable receptor-like protein kinase At1g80640;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At1g80640; ORFNames=T21F11.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Goodman H.M., Gallant P., Keifer-Higgins S., Rubenfield M., Church G.M.;
RT "A 37.5 Kb sequence from Arabidopsis thaliana chromosome I.";
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0V7T5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0V7T5-2; Sequence=VSP_040171, VSP_040172;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA98917.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF27131.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U53501; AAA98917.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC018849; AAF27131.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36430.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36431.1; -; Genomic_DNA.
DR EMBL; BT026485; ABH04592.1; -; mRNA.
DR PIR; F96838; F96838.
DR RefSeq; NP_001077858.1; NM_001084389.1. [Q0V7T5-2]
DR RefSeq; NP_178179.2; NM_106712.5. [Q0V7T5-1]
DR AlphaFoldDB; Q0V7T5; -.
DR SMR; Q0V7T5; -.
DR BioGRID; 29621; 1.
DR IntAct; Q0V7T5; 1.
DR STRING; 3702.AT1G80640.1; -.
DR PaxDb; Q0V7T5; -.
DR PRIDE; Q0V7T5; -.
DR ProteomicsDB; 232377; -. [Q0V7T5-1]
DR EnsemblPlants; AT1G80640.1; AT1G80640.1; AT1G80640. [Q0V7T5-1]
DR EnsemblPlants; AT1G80640.2; AT1G80640.2; AT1G80640. [Q0V7T5-2]
DR GeneID; 844403; -.
DR Gramene; AT1G80640.1; AT1G80640.1; AT1G80640. [Q0V7T5-1]
DR Gramene; AT1G80640.2; AT1G80640.2; AT1G80640. [Q0V7T5-2]
DR KEGG; ath:AT1G80640; -.
DR Araport; AT1G80640; -.
DR TAIR; locus:2198998; AT1G80640.
DR eggNOG; KOG1187; Eukaryota.
DR InParanoid; Q0V7T5; -.
DR OMA; VHHHDLN; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; Q0V7T5; -.
DR PRO; PR:Q0V7T5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0V7T5; baseline and differential.
DR Genevisible; Q0V7T5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Signal; Transferase; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..427
FT /note="Probable receptor-like protein kinase At1g80640"
FT /id="PRO_0000401361"
FT TOPO_DOM 23..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..427
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 149..413
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 275
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 155..163
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 223
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT VAR_SEQ 357..359
FT /note="AVP -> YLS (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040171"
FT VAR_SEQ 360..427
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040172"
SQ SEQUENCE 427 AA; 47373 MW; C5E3D82FF003112B CRC64;
MCLLRHLLPI WVLLGFFFFS TATKSQESSV VYVSGASPEI PISQASPRMG AQSPGPPIVK
VVLRQDLNKK ILIALIVSSS LLCVTVMFLV YLLLWRYRNM KNSFTGIKRK SDSVKSVTTK
PTVHKIDSVR KGTIPVYEYQ LLESATNKFS DSNVLSRGGR GCLYRACLDE KSSVTVKKLD
GGGETDIEKQ FETEVDWLAK IRHQNIVSLL GFCVYRQTSC IVYELMQNGS LESQLHGPSQ
GSGLTWQLRM KIAVDIARGL EYLHEHCHPP VVHRDLKSSS ILLDSDFNAK ISDFGFATVL
TTQNKNLIHK ASEDLLDGKV TDKNDVYSFG VILLELLLGK KSVEKPSSEP ESIVTWAVPK
LSDRANLPNI LDPAIKGTMD LKHLYQVAAV AVLCVQPEPS YRPLITDVLH SLIPLLPVEL
GGSLRIL
