Y1897_STAAM
ID Y1897_STAAM Reviewed; 453 AA.
AC Q99SY9;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Uncharacterized RNA methyltransferase SAV1897;
DE EC=2.1.1.-;
GN OrderedLocusNames=SAV1897;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01024}.
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DR EMBL; BA000017; BAB58059.1; -; Genomic_DNA.
DR RefSeq; WP_001147865.1; NC_002758.2.
DR AlphaFoldDB; Q99SY9; -.
DR SMR; Q99SY9; -.
DR World-2DPAGE; 0002:Q99SY9; -.
DR PaxDb; Q99SY9; -.
DR EnsemblBacteria; BAB58059; BAB58059; SAV1897.
DR KEGG; sav:SAV1897; -.
DR HOGENOM; CLU_014689_7_0_9; -.
DR OMA; FYAGDMK; -.
DR PhylomeDB; Q99SY9; -.
DR BioCyc; SAUR158878:SAV_RS10390-MON; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR11061; PTHR11061; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Methyltransferase;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..453
FT /note="Uncharacterized RNA methyltransferase SAV1897"
FT /id="PRO_0000162016"
FT ACT_SITE 411
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT BINDING 74
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 80
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 83
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 162
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 286
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT BINDING 315
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT BINDING 336
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT BINDING 384
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 453 AA; 51722 MW; F1D4E4C8A68A5E7B CRC64;
MQAIAKNDIK TGTVVDLTHE GHGVVKIDRF PIFIPQALIN EQIEYKIIKV KKNFAIGKLL
NINTRSENRV APPCIYYERC GGCQLQHLSY EAQLEMKKEQ VINLFQRKAH FDNSKINDTV
GMTDPWRYRN KSQIPVGKNE QNEVIMGFYR QRSHDIIDME SCLIQDSQHQ EVMNEVKSIL
KDLNVSIYQE QLKKGLMRHL VVRTGYHTDE MMIIFVTNGK KWPQKNAVVE KILDAFPNVT
SIKQNINDSH SNVIMGRQSI TLYGKDTIID QLTDSTFKIS DQSFYQINSE QTEKLYNKAI
EYAQLTGNEV VLDTYCGIGT IGLYMAPHAK HVYGVEVVPS SIEDAQQNAT INQCNNTTFV
CGKAEEVILQ WKAQGIKPDV VMVDPPRKGC DETFIQTLLT LEPKRIVYIS CNPATQQRDA
LLLAEKYQLE EVTPVDMFPQ TTHVETVALF NLK